PXD022498 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Meta-proteomics analysis of microbial ecosystem during the anaerobic digestion of chicken manure in biogas production farm |
Description | Meta-proteomics analysis approach in the application of biogas production from anaerobic digestion has many advantages that has not been fully uncovered yet. This study aims to investigate biogas production from a stable 2-stage chicken manure fermentation system in chemical and biological perspective. The diversity and functional protein changes from the 1st stage to 2nd stage is a good indication to expose the differential metabolic processes in anaerobic digestion. The highlight of identified functional proteins explain the causation of accumulated ammonia and carbon sources for methane production. Due to the ammonia stress and nutrient limitation, the hydrogenotrophic methanogenic pathway is adopted as indicative of meta-proteomics data involving the key methanogenic substrates (formate and acetate). Unlike traditional meta-genomic analysis, this study could provide both species names of microorganism and enzymes to directly point the generation pathway of methane and carbon dioxide in investigating biogas production of chicken manure. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-08 |
AnnouncementXML | Submission_2021-02-07_23:16:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tze-Kang Lam |
SpeciesList | scientific name: Methanoculleus sp.; NCBI TaxID: 90427; scientific name: Lactobacillus crispatus EX533959VC03; NCBI TaxID: 858653; scientific name: Clostridum phage CDHM6; NCBI TaxID: 2228361; |
ModificationList | monohydroxylated residue; iodoacetic acid derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-13 03:12:27 | ID requested | |
1 | 2021-02-07 23:13:10 | announced | |
⏵ 2 | 2021-02-07 23:16:24 | announced | 2021-02-08: Updated project metadata. |
3 | 2021-02-07 23:32:20 | announced | 2021-02-08: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Meta-Proteomics Analysis |
Chicken Manure |
Metabolic Pathway |
2-stage Fermentation System |
Ammonia Inhibition |
Contact List
Shih-Hsiung Wu |
contact affiliation | 128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan, IBC R712 |
contact email | shwu@gate.sinica.edu.tw |
lab head | |
Tze-Kang Lam |
contact affiliation | Institute of Biological Chemistry, Academia Sinica |
contact email | ltzekang@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022498
- Label: PRIDE project
- Name: Meta-proteomics analysis of microbial ecosystem during the anaerobic digestion of chicken manure in biogas production farm