PXD022459-2

PXD022459 is an original dataset announced via ProteomeXchange.

Title	Proteome changes in pepper (Capsicum annuum L.) leaves induced by the green peach aphid
Description	Aphid attack induces defense responses in plants activating several signaling cascades that led to the production of toxic, repellent or antinutritive compounds and the consequent reorganization of the plant primary metabolism. Pepper (Capsicum annuum L.) leaf proteomic response against Myzus persicae (Sulzer) has been investigated and analyzed by LC-MS/MS coupled with bioinformatics tools. Infestation with an initially low density (20 aphids/plant) of aphids restricted to a single leaf taking advantage of clip cages resulted in 6 differentially expressed proteins relative to control leaves (3 proteins at 2 days post-infestation and 3 proteins at 4 days post-infestation). Conversely, when plants were infested with a high density of infestation (200 aphids/plant) 140 proteins resulted differentially expressed relative to control leaves (97 proteins at 2 days post-infestation, 112 proteins at 4 days post-infestation and 105 proteins at 7 days post-infestation). The majority of proteins altered by aphid attack were involved in photosynthesis and photorespiration, oxidative stress, translation, protein folding and degradation and amino acid metabolism. Other proteins identified were involved in lipid, carbohydrate and hormone metabolism, transcription, transport, energy production and cell organization. However proteins directly involved in defense were scarce and were mostly downregulated in response to aphids. The unexpectedly very low number of regulated proteins found in the experiment with a low aphid density suggests an active mitigation of plant defensive response by aphids or alternatively an aphid strategy to remain undetected by the plant. Under a high density of aphids, pepper leaf proteome however changed significantly revealing nearly all routes of plant primary metabolism being altered. Photosynthesis was so far the process with the highest number of proteins being regulated by the presence of aphids. In general, at short times of infestation (2 days) most of the altered proteins were upregulated. However, at longer times of infestation (7 days) the protein downregulation prevailed. Proteins involved in plant defense and in hormone signaling were scarce and mostly downregulated.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:15:25.421.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victoria Florencio Ortiz
SpeciesList	scientific name: Capsicum annuum var. annuum; NCBI TaxID: 40321;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-11 02:37:20	ID requested
1	2020-11-18 06:28:53	announced
⏵ 2	2024-10-22 05:15:25	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Plant proteomics, plant defense, plant-aphid interaction, LC-MS/MS, biotic stress

Jose Luis Casas
contact affiliation	Unidad Asociada CSIC-UA IPAB. Instituto Universitario de Investigación CIBIO (Centro Iberoamericano de la Biodiversidad), University of Alicante.
contact email	jl.casas@ua.es
lab head
Victoria Florencio Ortiz
contact affiliation	I. U. CIBIO. Universty of Alicante.
contact email	victoria.florencio@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD022459

PRIDE project URI

• PRIDE
  1. PXD022459
     1. Label: PRIDE project
     2. Name: Proteome changes in pepper (Capsicum annuum L.) leaves induced by the green peach aphid