PXD022432-1

PXD022432 is an original dataset announced via ProteomeXchange.

Title	Nucleolin rescues TDP-43 toxicity in yeast and human cell models
Description	TDP-43 is a nuclear protein involved in pivotal processes, extensively studied for its implication in neurodegenerative disorders. TDP-43 cytosolic inclusions are a common neuropathologic hallmark in amyotrophic lateral sclerosis (ALS) and related diseases, and it is now established that TDP-43 misfolding and aggregation play a key role in their etiopathology. TDP-43 neurotoxic mechanisms are not yet clarified, but the identification of proteins able to modulate TDP-43-mediated damage may be promising therapeutic targets for TDP-43 proteinopathies. Here we show by the use of refined yeast models that the nucleolar protein nucleolin (NCL) acts as a potent suppressor of TDP-43 toxicity, restoring cell viability. We provide evidence that NCL co-expression is able to alleviate TDP-43-induced damage also in human cells, further supporting its beneficial effects in a more consistent pathophysiological context. Presented data suggest that NCL could promote TDP-43 nuclear retention, reducing the formation of toxic cytosolic TDP-43 aggregates.
HostingRepository	PRIDE
AnnounceDate	2021-03-26
AnnouncementXML	Submission_2021-03-25_23:10:52.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Stella
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-09 03:24:50	ID requested
⏵ 1	2021-03-25 23:10:53	announced

Dataset with its publication pending

submitter keyword: Neurodegenerative disorders, TDP-43 proteinopathies, ALS, FTD, nucleolin, nucleo-cytoplasmic transport, TMT, LC-HRMS/MS

Alessandro Bertoli
contact affiliation	Department of Biomedical Sciences, University of Padova, Italy CNR-Neuroscience Institute, Italy Padova Neuroscience center, University of Padova, Italy
contact email	alessandro.bertoli@unipd.it
lab head
Roberto Stella
contact affiliation	Istituto Zooprofilattico Sperimentale delle Venezie
contact email	rstella@izsvenezie.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022432
     1. Label: PRIDE project
     2. Name: Nucleolin rescues TDP-43 toxicity in yeast and human cell models