PXD022409-1

PXD022409 is an original dataset announced via ProteomeXchange.

Title	Maternally inherited piRNAs silence transposons during Drosophila embryogenesis
Description	The piRNA pathway controls transposon expression in animal germ cells, thereby ensuring genome stability over generations. piRNAs are maternally deposited and required for proper transposon silencing in adult offspring. However, a long-standing question in the field is the precise function of maternally deposited piRNAs and its associated factors during embryogenesis. Here, we probe the spatio-temporal expression patterns of several piRNA pathway components during early stages of development. Amongst those, factors required for transcriptional gene silencing (TGS) showed ubiquitous abundance in somatic and pole cells throughout the first half of embryogenesis. We further analysed the transcriptomes of various embryo stages and correlated these with the presence of selected chromatin marks. We found that a number of transposon families show bursts of transcription during early embryonic stages. Transposons heavily targeted by maternally deposited piRNAs accumulated repressive chromatin marks following their spike in expression. Furthermore, depletion of maternally deposited Piwi protein in early embryos resulted in increased expression of transposons targeted by inherited piRNAs and was accompanied by a strong loss of repressive chromatin marks at coding sequences. Overall, our data suggests a pivotal role for the piRNA pathway in transposon defence during Drosophila embryogenesis in somatic cells.
HostingRepository	PRIDE
AnnounceDate	2021-07-26
AnnouncementXML	Submission_2021-07-26_00:14:04.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Fabry
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-06 08:30:37	ID requested
⏵ 1	2021-07-26 00:14:04	announced
2	2023-11-14 08:51:46	announced	2023-11-14: Updated project metadata.

10.7554/ELIFE.68573;

submitter keyword: Drosophila, Embryogenesis, piRNA, TMT-MS

Gregory J Hannon
contact affiliation	Cancer Research UK Cambridge Institute University of Cambridge United Kingdom
contact email	greg.hannon@cruk.cam.ac.uk
lab head
Martin Fabry
contact affiliation	University of Cambridge
contact email	mhf27@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022409
     1. Label: PRIDE project
     2. Name: Maternally inherited piRNAs silence transposons during Drosophila embryogenesis