PXD022408-1

PXD022408 is an original dataset announced via ProteomeXchange.

Title	Cryo-EM structural analysis of FADD: Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate
Description	Uncovering the molecular architecture of the core FADD:Caspase-8 complex and how this is altered by regulatory partners, such as the cell death inhibitor c-FLIP, is essential to understand co-ordination of cell fate. Here, using electron microscopy, we visualize for the first time fulllength procaspase-8 in a complex with FADD. Our structural analysis reveals how the FADDnucleated tandem death effector domain (tDED) helical filament is required to correctly orientate procaspase-8 catalytic domains, enabling activation via anti-parallel dimerization.
HostingRepository	PRIDE
AnnounceDate	2021-02-08
AnnouncementXML	Submission_2021-02-08_00:59:01.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022408
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rebekah Jukes-Jones
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-06 07:03:52	ID requested
⏵ 1	2021-02-08 00:59:01	announced

Dataset with its publication pending

submitter keyword: Cell death. DISC, FADD, Caspase 8, c-Flip Complex, EM, Structure

Marion M. MacFarlane
contact affiliation	MRC Toxicology Unit, University of Cambridge, Hodgkin Building, Lancaster Road, Leicester, LE1 9HN, UK
contact email	mm2312@mrc-tox.cam.ac.uk
lab head
Rebekah Jukes-Jones
contact affiliation	MRC Toxicology Unit
contact email	rj396@mrc-tox.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022408

PRIDE project URI

• PRIDE
  1. PXD022408
     1. Label: PRIDE project
     2. Name: Cryo-EM structural analysis of FADD: Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate