PXD022382-2

PXD022382 is an original dataset announced via ProteomeXchange.

Title	Memo1 promotes FGF23-driven signaling by oxidative modulation of small Rho-GTPases and protein tyrosine phosphatase activity
Description	Memo is a copper-dependent redox enzyme modulating receptor tyrosine kinase signaling by unknown mechanisms (Meira et al., Nat Cell Biol 2004; MacDonald et al., Science Signal 2014). Memo1 deletion causes a phenotype partially resembling Klotho and Fgf23-deficient mice (Haenzi et al., FASEB J 2014; Moor et al., JBMR Plus 2018) and increases renal abundance of Rho-GTPase RhoA but prevents FGF23-dependent renal ERK phosphorylation and Rho-GTPase Rac1 activation (unpublished). To delineate potential effects of Memo’s redox function on FGF23-driven cellular signaling processes, we performed a redox proteomics screen which identified differently oxidized Rho-GTPase chaperone protein ARHGDIA/Rho-GDP dissociation inhibitor 1 (RhoGDI) as an interaction partner of Memo. RhoGDI is regulated by cysteine oxidation and phosphorylation. Here, we investigated the potential interaction between MEMO and RhoGDI by co-incubation of recombinant proteins MEMO and RhoGDI in cell-free environment to assess the Cys79 oxidation state of RhoGDI.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:26.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	dihydroxylated residue; cysTMT6plex reporter+balance reagent cysteine disulfide; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-05 06:09:49	ID requested
1	2023-10-24 10:27:47	announced
⏵ 2	2023-11-14 09:06:27	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:07:52	announced	2024-10-22: Updated project metadata.

Bartos K, Ramakrishnan SK, Braga-Lagache S, H, ä, nzi B, Durussel F, Prakash Sridharan A, Zhu Y, Sheehan D, Hynes NE, Bonny O, Moor MB, Renal FGF23 signaling depends on redox protein Memo1 and promotes orthovanadate-sensitive protein phosphotyrosyl phosphatase activity. J Cell Commun Signal, 17(3):705-722(2023) [pubmed]

submitter keyword: ARHGDIA, RhoGDI1, redox signaling,Memo1, cysteine oxidation

Matthias Moor
contact affiliation	University Hospital Bern
contact email	Matthias.moor@dbmr.unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022382
     1. Label: PRIDE project
     2. Name: Memo1 promotes FGF23-driven signaling by oxidative modulation of small Rho-GTPases and protein tyrosine phosphatase activity