PXD022374-1

PXD022374 is an original dataset announced via ProteomeXchange.

Title	Secretome analysis of human mammary gland fibroblasts and breast cancer-associated fibroblasts
Description	Cancer Associated Fibroblasts (CAF) are a dominant and critical cell type of the tumour microenvironment and can lead to breast cancer progression. TGF-β has been reported to influence fibroblast to myofibroblast activation, which is similar to cancer associated fibroblast phenotype. To understand the mechanism of TGF-β mediated CAF phenotype, we have performed a comparative proteomic analysis of conditioned media from CAF (isolated from breast cancer biopsy tissues) and normal mammary fibroblasts engineered to over-express TGF-β1. Liquid chromatography/ tandem mass spectrometry (LC ESI Q-TOF-MS/MS) assay of conditioned media and Venn analysis of the acquired data, revealed approximately 185 common proteins secreted by the three fibroblast types (CAF, normal mammary fibroblasts and TGF-β over expressing mammary fibroblasts). Among these, 12 proteins exclusively overlap between normal fibroblasts and CAF and 20 proteins exclusively overlap between CAF and TGF-β1 over-expressing fibroblasts. This analysis reveals interesting targets which may be important in activation phenotype of CAF and breast cancer progression.
HostingRepository	PRIDE
AnnounceDate	2025-08-26
AnnouncementXML	Submission_2025-08-26_01:33:04.989.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022374
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Armin Gandhi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Agilent MassHunter format; 6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-05 01:38:14	ID requested
⏵ 1	2025-08-26 01:33:05	announced

10.6019/PXD022374;

submitter keyword: fibroblast activation, TGF-β,Breast cancer progression, tumour microenvironment, conditioned medium, comparative proteomic analysis

Kondaiah Paturu
contact affiliation	Molecular Reproduction, Development and Genetics, GB01, Indian Institute of Science, India
contact email	paturu@alum.iisc.ac.in
lab head
Armin Gandhi
contact affiliation	INDIAN INSTITUTE OF SCIENCE
contact email	armingandhi@iisc.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022374
     1. Label: PRIDE project
     2. Name: Secretome analysis of human mammary gland fibroblasts and breast cancer-associated fibroblasts