PXD022306-1

PXD022306 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of Streptococcsus suis serotype 2 wild-type strain ZY05719 and stk-deficient strain by label-free mass spectrometry
Description	To identify potential substrates of STK and their target phosphorylation sites in SS2, phosphoproteomic analysis was performed. A comparison of phosphorylated peptides from the WT strain (ZY05719) to those in the mutant strain (Δstk) helped us to identify phosphopeptides that disappeared or decreased in the mutant strain Δstk and are likely substrates of STK. In total, 41 unique phosphopeptides from 31 proteins were identified in S. suis lysates. Among the identified phosphopeptides, 44 phosphorylation sites were identified: 16 on serine, 23 on threonine and 5 on tyrosine. The phosphopeptides were considered to be phosphorylated by STK. Eleven phosphopeptides corresponding to 9 proteins had decreased phosphorylation in the Δstk strain. Twenty phosphopeptides corresponding to 14 proteins were only identified in the wild-type strain ZY05719. Because of the level of phosphorylation changes, these proteins may be STK target proteins that are phosphorylated in vivo.
HostingRepository	iProX
AnnounceDate	2020-11-03
AnnouncementXML	Submission_2020-11-02_20:59:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weiyi Li
SpeciesList	scientific name: Streptococcus suis; NCBI TaxID: 1307;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-02 20:54:26	ID requested
⏵ 1	2020-11-02 20:59:44	announced

Dataset with its publication pending

submitter keyword: Streptococcus suis serotype 2, STK, Phosphoproteomics

Hongjie Fan
contact affiliation	Nanjing Agricultural University
contact email	fanhongjie2020@163.com
lab head
Weiyi Li
contact affiliation	Nanjing Agricultural University
contact email	liweiyinjau@163.com
dataset submitter

iProX dataset URI