PXD022283-1

PXD022283 is an original dataset announced via ProteomeXchange.

Title	Alterations in the CO2 availability induce alterations in the phospho-proteome of the cyanobacterium Synechocystis sp. PCC 6803
Description	Cyanobacteria are the only prokaryotes that perform plant-like oxygenic photosynthesis. They evolved an inorganic carbon-concentrating mechanism to adapt to low CO2 conditions. Quantitative phospho-proteomics was applied to analyze regulatory features during the acclimation to low CO2 conditions in the model cyanobacterium Synechocystis sp. PCC 6803. Overall, more than 2500 proteins were quantified, equivalent to approximately 70% of the Synechocystis theoretical proteome. Proteins with changing abundances correlated largely with mRNA expression levels. Functional annotation of the non-correlating proteins revealed an enrichment of key metabolic processes fundamental for maintaining cellular homeostasis. Furthermore, 105 phospho-proteins harboring over 200 site-specific phosphorylation events were identified. Subunits of the bicarbonate transporter BCT1 and the redox switch protein CP12 were among phospho-proteins with significantly reduced phosphorylation levels at lower CO2, whereas the serine/threonine protein kinase SpkC revealed increased phosphorylation levels. The corresponding spkC mutant was characterized and showed decreased ability to acclimate to low CO2 conditions. Possible phosphorylation targets of SpkC including a BCT1 subunit were identified by phospho-proteomics. Collectively, our study highlights the importance of post-transcriptional regulation of protein abundances as well as post-translational regulation by protein phosphorylation for the successful acclimation towards low CO2 conditions in Synechocystis and possibly among cyanobacteria.
HostingRepository	PRIDE
AnnounceDate	2021-07-14
AnnouncementXML	Submission_2021-07-13_22:24:10.218.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philipp Spaet
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-02 01:41:40	ID requested
⏵ 1	2021-07-13 22:24:12	announced

Sp, ä, t P, Barske T, Ma, č, ek B, Hagemann M, availability induce alterations in the phosphoproteome of the cyanobacterium Synechocystis sp. PCC 6803. New Phytol, 231(3):1123-1137(2021) [pubmed]

submitter keyword: cyanobacteria, Synechocystis sp. PCC 6803, bicarbonate transport, carbon acclimation, mutant, phosphorylation, protein kinase, SpkC

Martin Hagemann
contact affiliation	Institut für Biowissenschaften, Abteilung Pflanzenphysiologie, Universität Rostock, A.-Einstein-Str. 3, Rostock D-18059, Germany
contact email	martin.hagemann@uni-rostock.de
lab head
Philipp Spaet
contact affiliation	University Tuebingen Department of Quantitative Proteomics
contact email	philipp.spaet@uni-tuebingen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022283
     1. Label: PRIDE project
     2. Name: Alterations in the CO2 availability induce alterations in the phospho-proteome of the cyanobacterium Synechocystis sp. PCC 6803