PXD022275-1

PXD022275 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Arabidopsis early cold response phosphoproteomics
Description	Cold stress is one of the major limiting factors for global crop production. For survival at low temperature, plants need to sense the temperature changes in the surrounding environment. How plants sense and respond to the earliest drop in temperature is still not clearly understood. The plasma membrane and its adjacent extracellular and cytoplasmic sites are the first checkpoints for sensing temperature changes and subsequent events such as signal generation and solute transport. To understand how plants respond to early cold exposure, we have used a mass spectrometry-based phosphoproteomic method to study temporal changes in protein phosphorylation events in Arabidopsis membranes during 5 to 60 min of cold exposure. Phosphoproteomic analysis was carried out by using the following process. Arabidopsis membrane fraction was extracted from leaves. Trypsin digestion and phosphopeptide enrichment by HAMMOC method were performed, then purified peptide sample was analyzed by TripleTOF5600 (AB-SCIEX).
HostingRepository	jPOST
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-01_08:00:04.532.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Md Mostafa Kamal
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-01 22:18:53	ID requested
⏵ 1	2021-11-01 08:00:04	announced
2	2022-09-18 02:33:13	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Arabidopsis, cold response, membrane phosphoproteomics, stress perception, stress signaling, cold stress

submitter keyword: Arabidopsis, cold response, membrane phosphoproteomics, stress perception, stress signaling, cold stress

Contact List

Matsuo Uemura
lab head
Md Mostafa Kamal
contact affiliation	Faculty of Agriculture, Iwate University, Japan
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000993

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.biosciencedbc.jp/archive/jpostrepos/JPST000993