PXD022202-1

PXD022202 is an original dataset announced via ProteomeXchange.

Title	Mannose and mannobiose specific responses of insect associated cellulolytic Streptomyces.
Description	The cellulolytic insect symbiont bacterium, Streptomyces sp. SirexAA-E (SirexAA-E), is known to secrete a suite of Carbohydrate Active enZymes (CAZymes) for plant cell wall degradation in response to the available carbon sources. In this study, we sought to examine a poorly understood response of this bacterium to mannan, one of the major components of the plant cell wall, particularly in pine trees, the preferred host for SirexAA-E. SirexAA-E grew well on glucose, mannose, carboxymethyl cellulose (CMC), and locust bean gum (LBG) as sole carbon sources in the culture medium. The secreted proteins from each culture supernatant were tested for the polysaccharide-degrading ability, and the composition of secreted CAZymes in each culture supernatant were determined by LC-MS/MS. The results indicated that mannose, LBG, and CMC induced the secretion of mannan and cellulose-degrading enzymes. We found two α-1,2-mannosidases that were secreted during growth on mannose and LBG. By genomic analysis, we found a unique 12 bp palindromic sequence motif (5’-GACAACGTTGTC-3’) at 4 locations in the SirexAA-E genome, two of which were found upstream of genes encoding the above-mentioned α-1,2-mannosidases, along with a newly identified novel mannose and mannobiose responsive transcriptional regulator, SsManR. Furthermore, a transcriptional repressor that we previously showed was responsive to cellobiose, SsCebR, was determined to also use mannobiose as an effector ligand by in vitro binding assays. To test whether mannobiose induces the sets of genes under control of the two regulators, SsManR and SsCebR, SirexAA-E was grown on mannobiose, and the secretome composition was analyzed. As hypothesized, the composition of the mannobiose secretome combined sets of CAZymes found in both LBG and CMC secretomes, and so are likely under the regulation of both SsManR and SsCebR. To summarize, our findings support a transcriptional network that regulates mannose and LBG utilizing genes in SirexAA-E. Overall, this study defines a novel mannose regulatory transcriptional network that enables SirexAA-E to aggressively hydrolyze mannan-containing wood materials.
HostingRepository	PRIDE
AnnounceDate	2021-05-06
AnnouncementXML	Submission_2021-05-05_18:51:35.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taichi E. Takasuka
SpeciesList	scientific name: Streptomyces sp. SirexAA-E; NCBI TaxID: 862751;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-27 02:26:06	ID requested
⏵ 1	2021-05-05 18:51:36	announced

Dataset with its publication pending

submitter keyword: Cellulose utilization, Streptomyces sp. SirexAA-E, Glycoside hydrolases, Transcriptional regulation, Proteomics analysis

Taichi E Takasuka
contact affiliation	Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan
contact email	tendo.nd@gmail.com
lab head
Taichi E. Takasuka
contact affiliation	Research Faculty of Agriculture, Hokkaido University
contact email	tendo.nd@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022202
     1. Label: PRIDE project
     2. Name: Mannose and mannobiose specific responses of insect associated cellulolytic Streptomyces.