PXD022194-1

PXD022194 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics by use of a microfluidics chip.
Description	A microfluidics technology was implemented to the immunoaffinity purification process of MHC peptides in Ligandomics/Immunopeptidomics. The thus purified HLA peptides were analysed by LCMS with the nanoElute LC and TimsTOF Pro Mass Spectrometer from Bruker. The aim of the microfluidics implementation was to improve the sensitivity and robustness while also reducing antibody and other material requirements in the immunoaffinity purification protocol.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_16:30:50.970.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Branca
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-27 01:16:02	ID requested
⏵ 1	2022-02-16 16:30:51	announced

Feola S, Haapala M, Peltonen K, Capasso C, Martins B, Antignani G, Federico A, Pieti, ä, inen V, Chiaro J, Feodoroff M, Russo S, Rannikko A, Fusciello M, Koskela S, Partanen J, Hamdan F, T, ä, hk, ä SM, Yl, ö, sm, ä, ki E, Greco D, Gr, ö, nholm M, Kekarainen T, Eshaghi M, Gurvich OL, Yl, ä, -Herttuala S, M Branca RM, Lehti, ö J, Sikanen TM, Cerullo V, PeptiCHIP: A Microfluidic Platform for Tumor Antigen Landscape Identification. ACS Nano, 15(10):15992-16010(2021) [pubmed]

submitter keyword: Human, Ligandome, Immunopeptidome, microfluidics, chip, MHC-I, HLA-I, JY cell line, RCC, renal cell carcinoma, bladder cancer, patient derived organoid, ovarian cancer

Janne Lehtio
contact affiliation	Dept. Oncology-Pathology, Scilifelab, Karolinska Institutet, Stockholm, Sweden
contact email	janne.lehtio@ki.se
lab head
Rui Branca
contact affiliation	Clinical Proteomics Unit, Dep. of Oncology-Pathology
contact email	rui.mamede-branca@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD022194

PRIDE project URI

• PRIDE
  1. PXD022194
     1. Label: PRIDE project
     2. Name: Immunopeptidomics by use of a microfluidics chip.