PXD022153-1

PXD022153 is an original dataset announced via ProteomeXchange.

Title	A potential role of Gsdf-eEF1 complex in inhibiting germ cell proliferation: A protein interaction analysis in medaka (Oryzias latipes) from a proteomics perspective
Description	Gonadal soma-derived factor (gsdf) has been demonstrated as essential for testicular differentiation in medaka (Oryzias latipes). To understand the protein dynamics of Gsdf in spermatogenesis regulation, we used a His-tag “pull-down” assay coupled with shotgun LC-MS/MS to identify a group of potential interaction partners for Gsdf, which included cytoplasmic dynein light chain 2, eukaryotic polypeptide elongation factor 1 alpha (eEF1), and actin filaments in mature medaka testis. Alike the interaction with TGF-dynein critical for spermatogonial division in Drosophila melanogaster, the physical interaction of Gsdf & dynein and Gsdf & eEF1 were identified through a yeast 2-hybrid (Y2H) screening of an adult testis cDNA library using Gsdf as bait, and were verified by a paired Y2H assay. Co-immunoprecipitation of Gsdf and eEF1was defined in adult testes, as supporting the requirement of Gsdf and eEF1 interaction in testis development. Proteomics analysis and ultrastrutural observation showed that Gsdf deficiency activated eEF1-mediated protein synthesis and ribosome biogenesis, which in turn led to the differentiation of undifferentiated germ cell. Thus, our results provide a framework and new insight into the coordination of Gsdf (TGF and eEF1complex in the basic processes of germ cell proliferation, and transcriptional and translational control of sexual RNA, which may be fundamentally conserved across phyla during sex differentiation.
HostingRepository	PRIDE
AnnounceDate	2020-12-09
AnnouncementXML	Submission_2020-12-09_06:54:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guijun Guan
SpeciesList	scientific name: Oryzias latipes; NCBI TaxID: 8090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-23 02:46:53	ID requested
⏵ 1	2020-12-09 06:54:58	announced
2	2020-12-10 23:19:01	announced	2020-12-11: Updated publication reference for PubMed record(s): 33293461.

Dataset with its publication pending

submitter keyword: gsdf

eEF1

42Sp50

Guijun Guan
contact affiliation	Shanghai Ocean University
contact email	gjguan@shou.edu.cn
lab head
Guijun Guan
contact affiliation	Shanghai Ocean University
contact email	gjguan@shou.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022153
     1. Label: PRIDE project
     2. Name: A potential role of Gsdf-eEF1 complex in inhibiting germ cell proliferation: A protein interaction analysis in medaka (Oryzias latipes) from a proteomics perspective