PXD022146 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MigExpress discovers essential cell migration control genes in non-small cell lung cancer |
Description | Cell migration is an essential process in health and disease - especially in cancer metastasis. The difficulty to assess migration in high throughput presents a methodological hurdle - hence, only very few screens revealed factors controlling this important process. Here, we introduce MigExpress as a platform for the "identification of Migration control genes by differential Expression". MigExpress exploits the combination of in-depth molecular profiling and the robust quantitative analysis of migration capacity in a broad panel of samples and identifies migration-associated genes by their differential expression in slowly versus fast migrating cells. We applied MigExpress to non-small cell lung cancer (NSCLC), the most frequent cause of cancer mortality mainly due to metastasis. In 54 NSCLC cell lines, we comprehensively determined mRNA and protein expression. Correlating the transcriptome and proteome profiles with the quantified migration properties led to the discovery and validation of FLNC, DSE, CPA4, TUBB6 and BICC1 as migration control factors in NSCLC cells, which also negatively correlated with patient survival. Notably, FLNC was the least expressed filamin in NSCLC, but the only one controlling cell migration and correlating with patient survival and metastatic disease stage. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-20 |
AnnouncementXML | Submission_2021-04-20_09:24:00.180.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chen Meng |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-22 23:38:35 | ID requested | |
⏵ 1 | 2021-04-20 09:24:00 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cell migration, non-small cell lung cancer (NSCLC), proteomics, transcriptomics, Tandem mass tags (TMT), isobaric labeling |
Contact List
Christina Ludwig |
contact affiliation | Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University of Munich (TUM), Freising, Germany |
contact email | tina.ludwig@tum.de |
lab head | |
Chen Meng |
contact affiliation | Bavarian Center for Biomolecular Mass Spectrometry |
contact email | chen.meng@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022146
- Label: PRIDE project
- Name: MigExpress discovers essential cell migration control genes in non-small cell lung cancer