PXD022105 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila |
Description | In animal gonads, the PIWI-interacting RNA (piRNA) pathway guards genome integrity in part through the co-transcriptional gene silencing of transposon insertions. In Drosophila ovaries, piRNA-loaded Piwi detects nascent transposon transcripts and instructs heterochromatin formation through the Panoramix-induced co-transcriptional silencing (PICTS) complex, containing Panoramix, Nxf2 and Nxt1. Here, we report that the highly conserved dynein light chain LC8/Cut-up (Ctp) is an essential component of the PICTS complex. Loss of Ctp results in transposon de-repression and a reduction in repressive chromatin marks specifically at transposon loci. In turn, Ctp can enforce transcriptional silencing when artificially recruited to RNA and DNA reporters. We show that Ctp drives dimerisation of the PICTS complex through its interaction with conserved motifs within Panoramix. Artificial dimerisation of Panoramix bypasses the necessity for its interaction with Ctp, demonstrating that conscription of a protein from a ubiquitous cellular machinery has fulfilled a fundamental requirement for a transposon silencing complex. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-26 |
AnnouncementXML | Submission_2021-01-25_22:37:15.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Evelyn Eastwood |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | monohydroxylated residue; deaminated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-21 02:33:19 | ID requested | |
⏵ 1 | 2021-01-25 22:37:15 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Drosophila, piRNA |
Contact List
Gregory Hannon |
contact affiliation | Cancer Research UK- Cambridge Institute Li Ka Shing Centre Robinson way Cambridge CB2 0RE UK |
contact email | greg.hannon@cruk.cam.ac.uk |
lab head | |
Evelyn Eastwood |
contact affiliation | Cancer Research UK Cambridge Institute University of Cambridge |
contact email | evelyn.eastwood@cruk.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022105
- Label: PRIDE project
- Name: Dimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila