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PXD022103-1

PXD022103 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDimerisation of the PICTS complex via LC8/Cut-up drives co-transcriptional transposon silencing in Drosophila
DescriptionIn animal gonads, the PIWI-interacting RNA (piRNA) pathway guards genome integrity in part through the co-transcriptional gene silencing of transposon insertions. In Drosophila ovaries, piRNA-loaded Piwi detects nascent transposon transcripts and instructs heterochromatin formation through the Panoramix-induced co-transcriptional silencing (PICTS) complex, containing Panoramix, Nxf2 and Nxt1. Here, we report that the highly conserved dynein light chain LC8/Cut-up (Ctp) is an essential component of the PICTS complex. Loss of Ctp results in transposon de-repression and a reduction in repressive chromatin marks specifically at transposon loci. In turn, Ctp can enforce transcriptional silencing when artificially recruited to RNA and DNA reporters. We show that Ctp drives dimerisation of the PICTS complex through its interaction with conserved motifs within Panoramix. Artificial dimerisation of Panoramix bypasses the necessity for its interaction with Ctp, demonstrating that conscription of a protein from a ubiquitous cellular machinery has fulfilled a fundamental requirement for a transposon silencing complex.
HostingRepositoryPRIDE
AnnounceDate2021-03-04
AnnouncementXMLSubmission_2021-03-04_14:07:59.468.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEvelyn Eastwood
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; deaminated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-21 02:33:03ID requested
12021-03-04 14:07:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: drosophila, piRNA
Contact List
Gregory Hannon
contact affiliationCancer Research UK Cambridge Institute University of Cambridge Li Ka Shing Centre Robinson Way Cambridge CB2 0RE UK
contact emailgreg.hannon@cruk.cam.ac.uk
lab head
Evelyn Eastwood
contact affiliationCancer Research UK Cambridge Institute University of Cambridge
contact emailevelyn.eastwood@cruk.cam.ac.uk
dataset submitter
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Dataset FTP location
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