PXD022067-1

PXD022067 is an original dataset announced via ProteomeXchange.

Title	The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes
Description	Post-translational methylation plays a crucial role in regulating and optimizing protein function. Protein histidine methylation, occurring as the two isomers 1- and 3- methylhistidine (1MH and 3MH), was first reported five decades ago, but remains largely unexplored. Here we report that METTL9 is a broad-specificity methyltransferase (MTase) that mediates the formation of the majority of 1MH present in mouse and human proteomes. The minimal requirement for METTL9-catalyzed methylation is a His-x-His (HxH) motif, where "x" is preferably a small amino acid, allowing METTL9 to methylate a number of HxH-containing proteins. Here, we show that the immunomodulatory protein S100A9 and the NDUFB3 subunit of mitochondrial respiratory Complex I are methylated at HxH motif using MALDI-TOF MS.
HostingRepository	PRIDE
AnnounceDate	2020-11-23
AnnouncementXML	Submission_2020-11-23_07:28:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022067
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Takehiro Suzuki
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; 1'-methyl-L-histidine
Instrument	Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-19 03:54:12	ID requested
⏵ 1	2020-11-23 07:28:14	announced

Dataset with its publication pending

submitter keyword: Metylation Histidine METTL9

Naoshi Dohmae
contact affiliation	Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact email	dohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliation	RIKEN Center for Sustainable Resource Science
contact email	takehirosuzuki@riken.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022067
     1. Label: PRIDE project
     2. Name: The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes