PXD022058 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chemically induced cereblon (CRBN) degradation: efficacy of recruiting CRBN in comparison to VHL |
Description | As small molecule degrader technology has developed, it has raised the question of whether E3 ligases could be induced to self-ubiquitinate. To examine this question, we generated six CRBN-CRBN degraders (also referred to as homo-PROTACs) and six CRBN-VHL degraders (hetero-PROTACs). From these compounds we identified two potent and selective CRBN degraders (ZXH-4-130 and ZXH-4-137), both of which are CRBN-VHL compounds. Comparison of these compounds to previously reported small molecule CRBN degraders further validated our observation that CRBN-VHL compounds are more potent degraders of CRBN than CRBN-CRBN compounds. |
HostingRepository | PRIDE |
AnnounceDate | 2021-07-30 |
AnnouncementXML | Submission_2021-07-29_23:43:25.518.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Eric Fischer |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-19 03:52:49 | ID requested | |
⏵ 1 | 2021-07-29 23:43:25 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cereblon (CRBN) |
von Hippel-Lindau (VHL) protein |
E3 ubiquitin ligases |
small molecule degraders |
PROTACs |
targeted protein degradation |
chemical probe |
chemical biology |
Contact List
Eric Fischer |
contact affiliation | Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA |
contact email | eric_fischer@dfci.harvard.edu |
lab head | |
Eric Fischer |
contact affiliation | Dana-Farber Cancer Institute |
contact email | eric_fischer@dfci.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD022058 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022058
- Label: PRIDE project
- Name: Chemically induced cereblon (CRBN) degradation: efficacy of recruiting CRBN in comparison to VHL