PXD022056-1

PXD022056 is an original dataset announced via ProteomeXchange.

Title	Label-free mass spectrometry analysis of laminin with induced oxidations and chlorination’s facilitated with hypochlorous acid and myeloperoxidase treatments.
Description	Basement membranes are specialized extracellular matrices that underlie arterial wall endothelial cells, with laminin being a key structural and biologically-active component. Hypochlorous acid (HOCl), a potent oxidizing and chlorinating agent, is formed in vivo at sites of inflammation, via the enzymatic action of myeloperoxidase (MPO), released by activated leukocytes. Murine Laminin was treated with two oxidative systems to mimic the exposure of MPO-derived HOCl in atherosclerotic condition. Sites of oxidation and chlorination on isolated laminin-111 were detected and quantified by mass spectrometry. An increased number of modifications was detected with increasing oxidant exposure. HOCl oxidised 30 (of 56 total) Met and 7 (of 24) Trp-residues and chlorinated 33 (of 99) Tyr-residues; three Tyr-residues were dichlorinated. An additional 8 Met- and 10 Trp-oxidations, 14 chlorinations, and 18 dichlorinations were detected on Tyr-residues with the MPO system when compared to reagent HOCl. These data indicate that laminin is extensively modified by MPO-derived oxidants, with structural and functional changes. These modifications, and compromised cell-matrix interactions, may promote endothelial cell dysfunction, weaken the structure of atherosclerotic lesions, and enhance lesion rupture.
HostingRepository	PRIDE
AnnounceDate	2025-11-05
AnnouncementXML	Submission_2025-11-05_09:52:56.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adelina Rogowska-Wrzesinska
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; chlorinated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-19 03:52:33	ID requested
⏵ 1	2025-11-05 09:52:57	announced

Nybo T, Dieterich S, Gamon LF, Chuang CY, Hammer A, Hoefler G, Malle E, Rogowska-Wrzesinska A, Davies MJ, Chlorination and oxidation of the extracellular matrix protein laminin and basement membrane extracts by hypochlorous acid and myeloperoxidase. Redox Biol, 20():496-513(2019) [pubmed]

10.1016/j.redox.2018.10.022;

submitter keyword: ECM,LC-MS/MS, oxidation, extracellular matrix proteins, inflammation., chlorination, laminin

Adelina Rogowska-Wrzesinska
contact affiliation	University of Southern Denmark Department of biochemistry and molecular biology Protein research group
contact email	Adelinar@bmb.sdu.dk
lab head
Adelina Rogowska-Wrzesinska
contact affiliation	University of Southern Denmark
contact email	adelinar@bmb.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022056
     1. Label: PRIDE project
     2. Name: Label-free mass spectrometry analysis of laminin with induced oxidations and chlorination’s facilitated with hypochlorous acid and myeloperoxidase treatments.