PXD022029-1

PXD022029 is an original dataset announced via ProteomeXchange.

Title	Rhizobium leguminosarum glutathione peroxidase is essential for oxidative stress resistance and efficient nodulation
Description	Glutathione plays a tremendous role in regulating the homeostasis of redox state, and appears to be essential for proper development of the root nodules. Glutathione peroxidase catalyzes the reduction of peroxides by oxidation of GSH to oxidized GSH (GSSG), which can in turn be reduced by glutathione reductase (GR). However, it has not been determined whether the R. leguminosarum Gpx or GR is required during symbiotic interactions with pea. To characterise the role of glutathione-dependent enzymes in the symbiotic process, single and double mutants were made in gpxA and gorA genes. All the mutations did not affect the growth of R. leguminosarum, but they increased the sensitivity of R. leguminosarum strains to H2O2. The gorA mutant can induce the formation of normal nodules, while the gpxA single and double mutants exhibited an abnormal nodulation phenotype coupled to more than 50% reduction in nitrogen-fifixing capacity, this defect in nodulation was characterized by the formation of undeveloped and ineffective nodules. In addition, the gorA and gpxA double mutant was severely impaired in rhizosphere colonization. LC-MS/MS analysis quantitative proteomics techniques were employed to compare differential gpxA mutant root bacteroids in response to the wild type infection. Sixty differentially expressed proteins were identified including seven up-regulated and twenty down-regulated proteins. By sorting the down-regulated proteins according to metabolic function, eight proteins were transporter protein, seven proteins were dehydrogenases and deoxygenases. Moreover, three down-regulation proteins are directly involved in nodule process.
HostingRepository	PRIDE
AnnounceDate	2020-11-16
AnnouncementXML	Submission_2020-11-16_07:52:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guojun Cheng
SpeciesList	scientific name: Rhizobium leguminosarum bv. viciae; NCBI TaxID: 387;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-16 03:46:15	ID requested
⏵ 1	2020-11-16 07:52:15	announced

Dataset with its publication pending

submitter keyword: Rhizobium leguminosarum

glutathione peroxidase

antioxidant function

symbiotic nitrogen fixation

quantitative proteomics

Guojun Cheng
contact affiliation	College of Life Sciences, South-Central University for Nationalities
contact email	chengguojun@mail.scuec.edu.cn
lab head
Guojun Cheng
contact affiliation	College of Life Sciences, South-Central University for Nationalities
contact email	chengguojun@mail.scuec.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022029
     1. Label: PRIDE project
     2. Name: Rhizobium leguminosarum glutathione peroxidase is essential for oxidative stress resistance and efficient nodulation