PXD021998-1

PXD021998 is an original dataset announced via ProteomeXchange.

Title	Proteomic dissection of the impact of environmental exposures on mouse seminal vesicle function
Description	The seminal vesicles are an integral part of the male accessory gland system, and are responsible for producing a complex array of bioactive moieties that support gamete function and promote reproductive success. However, despite their physiological significance, the biology of the seminal vesicles remains poorly defined. Here, we exploit an established reproductive toxicant model, where mice were administered acrylamide (25 mg/kg bw/day) or control each morning for 5 consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection, to complete the first proteomic assessment of mouse seminal vesicles. A total of 5,013 proteins were identified in the core seminal vesicle proteome with bioinformatics analysis of identifying cell proliferation, transcript/protein production and cellular death and survival pathways as prominent biological processes. Comparison of our acrylamide to control seminal vesicle proteome revealed 311 differentially regulated (-1.5 ≤ FC ≥ 1.5, p ≤ 0.05, 205 up-regulated, 106 down-regulated) proteins with immunoblotting analysis confirming proteomic data. Pathways that initiate protein synthesis to promote cellular survival were prominent amongst the dysregulated cellular pathways and RICTOR (Z-score = -3.64, p = 6.69E-07) was a top-ranked upstream driver. Oxidative stress was implicated as a contributor to protein changes with acrylamide leading to a significant increase in 8-OHdG in epithelial cells (5-fold increase, p = 0.016). Direct effects of acrylamide on seminal vesicle function were observed through a reduction in seminal vesicle secretion weight and overall protein content. Together these finding support the emerging concept that in addition to sperm, paternal exposures exert significant influence on the seminal vesicles, and identify new pathways to develop targeted interventions with which to manipulate fertility and reproductive outcomes.
HostingRepository	PRIDE
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-14_08:19:30.060.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021998
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Skerrett-Byrne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-15 01:16:34	ID requested
⏵ 1	2021-09-14 08:19:30	announced

Skerrett-Byrne DA, Trigg NA, Bromfield EG, Dun MD, Bernstein IR, Anderson AL, Stanger SJ, MacDougall LA, Lord T, Aitken RJ, Roman SD, Robertson SA, Nixon B, Schjenken JE, Proteomic Dissection of the Impact of Environmental Exposures on Mouse Seminal Vesicle Function. Mol Cell Proteomics, 20():100107(2021) [pubmed]

submitter keyword: Seminal Vesicle, Proteomics, Acrylamide

John Schjenken
contact affiliation	Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, The University of Newcastle, University Drive, Callaghan, NSW 2308, Australia; Hunter Medical Research Institute, Pregnancy and Reproduction Program, New Lambton Heights, NSW 2305, Australia
contact email	John.Schjenken@newcastle.edu.au
lab head
David Skerrett-Byrne
contact affiliation	The University of Newcastle
contact email	David.Skerrett-Byrne@newcastle.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021998
     1. Label: PRIDE project
     2. Name: Proteomic dissection of the impact of environmental exposures on mouse seminal vesicle function