PXD021992-1

PXD021992 is an original dataset announced via ProteomeXchange.

Title	Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings
Description	Protein phosphorylation and ubiquitination are two of the most abundant forms of post-translational modifications in eukaryotes, regulated by thousands of protein kinases, phosphatases, E3 ubiquitin ligases, and ubiquitin proteases. Although previous studies have catalogued several ubiquitinated proteins in plants (Walton et al., 2016), few membrane-localized proteins have been identified. Receptor kinases (RKs) initiate phosphorylation signal relays that regulate plant growth, development, and stress responses. While the regulatory role of phosphorylation on protein kinase function is well-documented (Couto and Zipfel, 2016), considerably less is known about the role of ubiquitination on protein kinase function, even though protein turnover is critical to their signaling competence and cellular homeostasis. Here we describe the large-scale identification of ubiquitination sites on Arabidopsis proteins associated with or integral to the plasma membrane, including over 100 protein kinases.
HostingRepository	PRIDE
AnnounceDate	2021-02-01
AnnouncementXML	Submission_2021-01-31_22:22:35.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021992
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	FRank Menke
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	L-methionine sulfoxide; ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-15 01:15:56	ID requested
⏵ 1	2021-01-31 22:22:35	announced

Dataset with its publication pending

submitter keyword: Ubiquitin, Arabidopsis, Membrane-associated, Immunity, plant

Frank L.H. Menke
contact affiliation	The Sainsbury laboratory University of East Anglia Norwich
contact email	Frank.Menke@tsl.ac.uk
lab head
FRank Menke
contact affiliation	the Sainsbury laboratory
contact email	Frank.Menke@tsl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021992
     1. Label: PRIDE project
     2. Name: Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings