PXD021970-1

PXD021970 is an original dataset announced via ProteomeXchange.

Title	Plasmodium berghei and Plasmodium falciparum LC-MS/MS
Description	Previously published data indicated that PgCHT2 forms a high molecular weight (HMW), reduction-sensitive complex; data suggested that the binding partner is the ookinete-produced von Willebrand A-domain-containing protein, WARP. To test the hypothesis that transgenic P.berghei ookinete-produced PfCHT1 could form a high molecular homo-multimer or, alternatively, could interact with P. berghei ookinete-produced proteins to produce a HMW hetero-multimer, we created a chimeric P. berghei parasites expressing PfCHT1 to replace PbCHT1, allowing production of large numbers of PfCHT1-expressing ookinetes.Mass spectrometry analysis was carried out on the chitin affinity pulled down proteins from the Pb-PfCHT1(r) ookinete culture supernatant.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_10:07:11.130.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joseph Vinetz
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-14 01:34:24	ID requested
⏵ 1	2021-09-09 10:07:12	announced

Patra KP, Kaur H, Kolli SK, Wozniak JM, Prieto JH, Yates JR, Gonzalez DJ, Janse CJ, Vinetz JM, Ookinete-Secreted Protein Complex Involved in Mosquito Midgut Invasion. Front Cell Infect Microbiol, 10():615343(2020) [pubmed]

submitter keyword: Plasmodium berghei and Plasmodium falciparum,Q-Exactive Plus (Thermo Fisher Scientific) LC MS/MS system

Prof. Jospeh M. Vinetz
contact affiliation	MD, FACP, FIDSA, FASTMH, BS Professor of Medicine (Infectious Diseases) and Anthropology, Yale University
contact email	jospeh.vinetz@yale.edu
lab head
Joseph Vinetz
contact affiliation	MD, FACP, FIDSA, FASTMH, BS, Professor of Medicine (Infectious Diseases) and Anthropology, Yale University
contact email	joseph.vinetz@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021970

PRIDE project URI

• PRIDE
  1. PXD021970
     1. Label: PRIDE project
     2. Name: Plasmodium berghei and Plasmodium falciparum LC-MS/MS