PXD021897-1

PXD021897 is an original dataset announced via ProteomeXchange.

Title	Drosophila Sex Peptide Controls the Assembly of Lipid Microcarriers in Seminal Fluid
Description	Seminal fluid plays an essential role in promoting male reproductive success and modulating female physiology and behaviour. In the fruit fly, Drosophila melanogaster, Sex Peptide (SP) is the best-characterised protein mediator of these effects. It is secreted from the paired male accessory glands (AGs), which, like the mammalian prostate and seminal vesicles, generate most of the seminal fluid contents. After mating, SP binds to spermatozoa and is retained in the female sperm storage organs. It is gradually released by proteolytic cleavage and induces several long-term post-mating responses including increased ovulation, elevated feeding and reduced receptivity to remating, primarily signalling through the SP receptor (SPR). We demonstrate a previously unsuspected SPR-independent function for SP. We show that, in the AG lumen, SP and secreted proteins with membrane-binding anchors are carried on abundant, large neutral lipid-containing microcarriers, also found in other SP-expressing Drosophila species. These microcarriers are transferred to females during mating, where they rapidly disassemble. Remarkably, SP is a key microcarrier assembly and disassembly factor. Its absence leads to major changes in the seminal proteome transferred to females upon mating. Males expressing non-functional SP mutant proteins that affect SP binding to and release from sperm in females also do not produce normal microcarriers, suggesting that this male-specific defect contributes to the resulting widespread abnormalities in ejaculate function. Our data reveal a novel role for SP in formation of seminal macromolecular assemblies, which may explain the presence of SP in Drosophila species that lack the signalling functions seen in D. melanogaster. In this experiment we assessed the effect of SP loss-of-function on the transferred seminal proteome.
HostingRepository	PRIDE
AnnounceDate	2021-01-26
AnnouncementXML	Submission_2021-01-26_04:45:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021897
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Charles
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-08 06:51:31	ID requested
⏵ 1	2021-01-26 04:45:57	announced

Dataset with its publication pending

submitter keyword: Reproduction, secretion, seminal proteins, triacylglycerides, Sex Peptide, Drosophila

Clive Wilson
contact affiliation	Department of Physiology, Anatomy and Genetics, University of Oxford
contact email	clive.wilson@dpag.ox.acuk
lab head
Philip Charles
contact affiliation	University of Oxford
contact email	philip.charles@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021897
     1. Label: PRIDE project
     2. Name: Drosophila Sex Peptide Controls the Assembly of Lipid Microcarriers in Seminal Fluid