PXD021884-1

PXD021884 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Analysis of an Alexander Disease Murine Model with Severe Reactive Gliosis
Description	We performed label-free micro-data-independent acquisition (µDIA) to assay brain proteins from a severe model of Alexander disease (AxD, lethal at postnatal day ~35). Of the 5,005 proteins quantified, we observed upregulation of adipocytokine signaling, PPAR, insulin resistance, and glutathione pathways with concomitant downregulation of platelet activation, ether lipid synthesis, and steroid biosynthesis pathways. To validate these DIA results, we setup a targeted parallel reaction monitoring-mass spectrometry assay (PRM-MS) for selected proteins with altered relative concentrations. The PRM-MS assays confirmed the upward or downward relative concentration differences in 16/16 (100%) of the proteins targeted, with 15/16 (94%) of these proteins reaching statistical significance (P-value < 0.05).
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:36:32.434.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Heaven
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-08 00:07:26	ID requested
⏵ 1	2024-10-07 13:36:33	announced

10.1016/j.mcpro.2021.100180;

Heaven MR, Herren AW, Flint DL, Pacheco NL, Li J, Tang A, Khan F, Goldman JE, Phinney BS, Olsen ML, Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis. Mol Cell Proteomics, 21(1):100180(2022) [pubmed]

submitter keyword: reactive gliosis,Alexander disease, parallel reaction monitoring, data-independent acquisition, brain proteome

Michelle L Olsen
contact affiliation	Virginia Tech School of Neuroscience Life Sciences Building (LS 1) Room 213, Lab 212 Blacksburg VA, 24061
contact email	molsen1@vt.edu
lab head
Michael Heaven
contact affiliation	CSO
contact email	mheaven@vulcanbiosciences.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD021884

PRIDE project URI

• PRIDE
  1. PXD021884
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic Analysis of an Alexander Disease Murine Model with Severe Reactive Gliosis