PXD021874 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Glycoproteomic analysis of Haloferax volcanii strains H53, DaglB, and Dagl15 |
Description | An in-depth glycoproteomic analysis of Haloferax volcanii has been performed, comparing the wildtype H53 with knockout mutants of aglB and agl15. While AglB-dependent glycosylation has been described to occur under standard culture conditions, the Agl15-dependent glycosylation pathway has previously been described to be only active under low-salt conditions. In this dataset, the largest archaeal glycoproteome could be identified with more than 40 N-glycoproteins and more than 100 N-glycopptides. It reveals not only that both glycosylation pathways can be active under the same conditions but also that the same glycosites can be modified by different glycans from both glycosylation pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-20 |
AnnouncementXML | Submission_2021-06-20_14:13:45.029.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Schulze |
SpeciesList | scientific name: Haloferax volcanii; NCBI TaxID: 2246; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-07 01:18:37 | ID requested | |
⏵ 1 | 2021-06-20 14:13:45 | announced | |
Publication List
Schulze S, Pfeiffer F, Garcia BA, Pohlschroder M, Comprehensive glycoproteomics shines new light on the complexity and extent of glycosylation in archaea. PLoS Biol, 19(6):e3001277(2021) [pubmed] |
Keyword List
submitter keyword: glycosylation, archaea, Haloferax volcanii, glycoproteomics |
Contact List
Mechthild Pohlschroder |
contact affiliation | University of Pennsylvania Department of Biology |
contact email | pohlschr@sas.upenn.edu |
lab head | |
Stefan Schulze |
contact affiliation | University of Pennsylvania |
contact email | sschulze@sas.upenn.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD021874 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021874
- Label: PRIDE project
- Name: Glycoproteomic analysis of Haloferax volcanii strains H53, DaglB, and Dagl15