PXD021857-1

PXD021857 is an original dataset announced via ProteomeXchange.

Title	High-density monitoring of tyrosine phosphorylation signaling targets in human tumors using internal standard triggered targeted quantitation
Description	SureQuant pTyr, a targeted, tyrosine phosphorylation (pTyr) mass spectrometry method, was applied to profile the pTyr signatures of human colorectal tumor samples. Using internal standard guided acquisition with the SureQuant acquisition mode on the Oribtrap Exploris 480 mass spectrometer (Thermo Scientific), the real-time detection of trigger peptides during analysis initiates the sensitive and selective quantitation of endogenous pTyr targets. This framework allows for reliable quantification of several hundred commonly dysregulated pTyr targets with high quantitative accuracy and improves the robustness and usability of targeted acquisition.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:24:54.481.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauren Stopfer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-06 01:20:20	ID requested
⏵ 1	2022-02-16 08:24:57	announced
2	2024-10-22 05:32:18	announced	2024-10-22: Updated project metadata.

Stopfer LE, Flower CT, Gajadhar AS, Patel B, Gallien S, Lopez-Ferrer D, White FM, High-Density, Targeted Monitoring of Tyrosine Phosphorylation Reveals Activated Signaling Networks in Human Tumors. Cancer Res, 81(9):2495-2509(2021) [pubmed]

submitter keyword: Targeted, cancer, phosphorylation, signaling, colorectal

Forest White
contact affiliation	Koch Institute for Integrate Cancer Research & Department of Biological Engineering, MIT
contact email	fwhite@mit.edu
lab head
Lauren Stopfer
contact affiliation	MIT
contact email	lstopfer@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD021857

PRIDE project URI

• PRIDE
  1. PXD021857
     1. Label: PRIDE project
     2. Name: High-density monitoring of tyrosine phosphorylation signaling targets in human tumors using internal standard triggered targeted quantitation