PXD021848-1

PXD021848 is an original dataset announced via ProteomeXchange.

Title	Proteomics of anatomical sections of the gut of Nosema-infected Western honey bee (Apis mellifera) reveals different early responses to Nosema spp. isolates
Description	We aim to evaluate the effects of four Nosema spores’ isolates, (i) and (ii) N. ceranae isolated from A. mellifera hosts from two different geographical origins, (iii) N. ceranae from A. cerana host and (iv) N. apis from A. mellifera, on the A. mellifera on gut proteomics at the early stage of infection. To dissect the molecular mechanism responsible of the susceptibility of A. mellifera to Nosema, we investigated by high-resolution proteomics (LC-ESI-MS/MS) and differential label-free quantification of proteins (LFQ) the molecular cross-talk existing between different species and isolates of N. apis and N. ceranae, and the targetted gut tissue of A. mellifera. To reach the objectives of this study, we performed a bottom-up proteomic analysis on the different anatomical sections of the gut tissue (esophagus, crop, midgut, ileum and rectum) at an early stage of the exposition to Nosema spores (4 days). Then, we focused on the midgut, the region targeted by Nosema sposres for germination and, as we found out, the second region with the highest load of Nosema proteins, after the rectum, to perform differential quantitative proteomic analyses and acquire series of up- and down-regulated proteins. We discussed the different pathways observed to be impacted by different Nosema species and isolates with a main focus on the deregulated metabolic and response to stimuli processes.
HostingRepository	PRIDE
AnnounceDate	2020-11-25
AnnouncementXML	Submission_2020-11-25_07:36:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Voisin
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460; scientific name: Nosema apis; NCBI TaxID: 35231; scientific name: Nosema ceranae; NCBI TaxID: 40302;
ModificationList	S-pyridylethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 14:04:35	ID requested
⏵ 1	2020-11-25 07:36:07	announced

Dataset with its publication pending

submitter keyword: Apis mellifera, Nosema ceranae, Nosema apis, digestive tract, proteomics

Philippe Bulet
contact affiliation	CR University Grenoble Alpes, Institute for Advanced Biosciences, Inserm U1209, CNRS UMR 5309, Grenoble, France
contact email	philippe.bulet@univ-grenoble-alpes.fr
lab head
Sebastien Voisin
contact affiliation	Plateforme BioPark of Archamps
contact email	sebastien.voisin@biopark-archamps.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021848
     1. Label: PRIDE project
     2. Name: Proteomics of anatomical sections of the gut of Nosema-infected Western honey bee (Apis mellifera) reveals different early responses to Nosema spp. isolates