PXD021832-1

PXD021832 is an original dataset announced via ProteomeXchange.

Title	Proteomic approache of the response to iron-limited condition in Staphylococcus lugdunensis
Description	Staphylococcus lugdunensis is a coagulase-negative Staphylococcus part of the commensal skin flora but emerge as an important opportunistic pathogen. Because iron limitation is a crucial stress during infectious process, we performed phenotypic study and compared proteomic profiles of this species incubated in absence and in presence of the iron chelator 2,2'-dipyridyl (DIP). No modification of cell morphology nor cell wall thickness were observed in presence of DIP. However iron-limitation condition promoted biofilm formation and reduced the ability to cope with oxidative stress (1 mM H2O2). In addition, S. lugdunensis N920143 cultured with DIP was significantly less virulent in the larvae of Galleria mellonella model of infection than that grown under standard conditions. We verified that these phenotypes were due to an iron limitation by complementation experiments with FeSO4. By mass spectrometry after trypsin digestion, we characterized the first iron-limitation stress proteome in S. lugdunensis. Among 1426 proteins identified, 349 polypeptides were differentially expressed. 222 were more and 127 less abundant in S. lugdunensis incubated in iron-limitation condition, and by RT-qPCR, some of the corresponding genes have been shown to be transcriptionally regulated. Our data revealed that proteins involved in iron metabolism and carriers were over-expressed, as well as several ABC transporters and polypeptides linked to cell wall metabolism. Conversely, enzymes playing a role in the oxidative stress response (especially catalase) were repressed. This phenotypic and global proteomic study allowed characterization of the response of S. lugdunensis to iron-limitation. We showed that iron-limitation promoted biofilm formation, but decrease the oxidative stress resistance that may, at least in part, explained the reduced virulence of S. lugdunensis observed under low iron condition.
HostingRepository	PRIDE
AnnounceDate	2020-10-13
AnnouncementXML	Submission_2020-10-13_05:35:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aubourg Marion
SpeciesList	scientific name: Staphylococcus lugdunensis N920143; NCBI TaxID: 1034809;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:55:07	ID requested
⏵ 1	2020-10-13 05:35:13	announced

Dataset with its publication pending

submitter keyword: Staphylococcus lugdunensis, iron limitation, proteomic, virulence, oxydative stress

Jean-Christophe GIARD
contact affiliation	Pr Jean-Christophe Giard Unité de Recherche Risques Microbiens (U2RM) Equipe "Antibio-résistance" Laboratoire de Microbiologie CHU Côte de Nacre 14033 Caen Cedex
contact email	jean-christophe.giard@unicaen.fr
lab head
Aubourg Marion
contact affiliation	PhD Student U2RM
contact email	marion.aubourg@unicaen.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021832
     1. Label: PRIDE project
     2. Name: Proteomic approache of the response to iron-limited condition in Staphylococcus lugdunensis