PXD021830-1

PXD021830 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Btk haemolymph stimulon
Description	The spore-forming bacterium Bacillus thuringiensis (Bt) of B. cereus group uses toxin-opened breaches at the insect midgut epithelium to infest the haemolymph where it rapidly propagates despite antimicrobial host defences to induce eventually host death by acute septicaemia. The response of Bt to haemolymph and the latter’s role in bacterial pathogenesis is an area that needs clarification. We report the proteome analysis of Bt subsp. kurstaki (Btk) haemolymph stimulon consisted of significant changes in 60 (34 up- and 26 down-regulated) differentially accumulated proteins (DAPs). Gene Ontology (GO) enrichment analysis revealed Btk haemolymph-responsive DAPs were mainly involved in glutamate metabolism, transketolase activity and ATP-dependent transmembrane transporters. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis disclosed that DAPs were highly enriched in enzymes related to the biosynthesis of ansamycins, a family of bacterial polyketides with antimicrobial activity. Interestingly, around 30% of DAPs were putative virulence factors, including polyketide synthase, ABC transporter, and cytotoxin. Collectively, our findings suggest Btk haemolymph stimulon could be partially driven bacterial survival and propagation within the haemolymph of infected insects, contributing to its remarkable success as entomopathogen.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_04:20:36.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Camilo Ayra-Pardo
SpeciesList	scientific name: Bacillus thuringiensis serovar kurstaki str. HD73; NCBI TaxID: 1279365;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:52:06	ID requested
⏵ 1	2021-11-02 04:20:37	announced

Dataset with its publication pending

submitter keyword: Insect pathogen, Bacillus thuringiensis, TMT, LC-MSMS

Yunchao Kan
contact affiliation	China-UK-NYNU-RRES Joint Laboratory of Insect Biology, Nanyang Normal University (NYNU), Nanyang 473061, Henan, People’s Republic of China
contact email	kanyunchao@163.com
lab head
Camilo Ayra-Pardo
contact affiliation	Nanyang Normal University
contact email	cayrapardo73@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021830
     1. Label: PRIDE project
     2. Name: Proteomics of Btk haemolymph stimulon