PXD021815-1

PXD021815 is an original dataset announced via ProteomeXchange.

Title	Overlap of NatA and IAP substrates implicates N-terminal acetyltion in protein stabilization
Description	SMAC/DIABLO and HTRA2 are mitochondrial proteins whose N-termina sequences, known as inhibitor of apoptosis binding motifs (IBMs), bind and activate ubiquitin ligases knwon as inhibitor of apoptosis proteins (IAPs), unleashing a cell´s poteintial.IBMs comprise a 4-residue, loose consensus sequence, and binding to IAPs requires an unmodified N-terminus. Closely related, IBM-like N-termini, are present in approximately 5% of human proteins. We show that suppression of the N-alpha-acetytransferase NatA truns these cryptic IBM-like sequences into very efficient IAP binders in cell lysates and in vitro, and ultimately also triggers apoptosis. Thus, N-terminal acetylation of IBM-like motifs in NatA substrates shields them from IAPs. This previously unrecognized relatonship suggests that N-terminal acetylation is generally protective against protein degradation in human cells. It also identifies IAPs as agents of a general quality control mechanism targeting unacetylated rogues in metazoans
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:45:53.993.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Bange
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:50:14	ID requested
⏵ 1	2024-10-07 10:45:54	announced

Dataset with its publication pending

submitter keyword: N-terminal acetylation, BIRC3, BIRC2, NatB,N-terminomics, NatA, apoptosis, XIAP, N-degron

Andrea Musacchio
contact affiliation	MPI of Molecular Physiology
contact email	andrea.musacchio@mpi-dortmund.mpg.de
lab head
Tanja Bange
contact affiliation	LMU Munich
contact email	tanja.bange@med.uni-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021815
     1. Label: PRIDE project
     2. Name: Overlap of NatA and IAP substrates implicates N-terminal acetyltion in protein stabilization