PXD021815 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Overlap of NatA and IAP substrates implicates N-terminal acetyltion in protein stabilization |
Description | SMAC/DIABLO and HTRA2 are mitochondrial proteins whose N-termina sequences, known as inhibitor of apoptosis binding motifs (IBMs), bind and activate ubiquitin ligases knwon as inhibitor of apoptosis proteins (IAPs), unleashing a cellĀ“s poteintial.IBMs comprise a 4-residue, loose consensus sequence, and binding to IAPs requires an unmodified N-terminus. Closely related, IBM-like N-termini, are present in approximately 5% of human proteins. We show that suppression of the N-alpha-acetytransferase NatA truns these cryptic IBM-like sequences into very efficient IAP binders in cell lysates and in vitro, and ultimately also triggers apoptosis. Thus, N-terminal acetylation of IBM-like motifs in NatA substrates shields them from IAPs. This previously unrecognized relatonship suggests that N-terminal acetylation is generally protective against protein degradation in human cells. It also identifies IAPs as agents of a general quality control mechanism targeting unacetylated rogues in metazoans |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_10:45:53.993.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tanja Bange |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-05 03:50:14 | ID requested | |
⏵ 1 | 2024-10-07 10:45:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: N-terminal acetylation, BIRC3, BIRC2, NatB,N-terminomics, NatA, apoptosis, XIAP, N-degron |
Contact List
Andrea Musacchio |
contact affiliation | MPI of Molecular Physiology |
contact email | andrea.musacchio@mpi-dortmund.mpg.de |
lab head | |
Tanja Bange |
contact affiliation | LMU Munich |
contact email | tanja.bange@med.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021815 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021815
- Label: PRIDE project
- Name: Overlap of NatA and IAP substrates implicates N-terminal acetyltion in protein stabilization