PXD021802-1
PXD021802 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization and Complete Genome Sequence of Privateer, a Highly Prolate Proteus mirabilis Podophage |
| Description | The Gram-negative bacterium Proteus mirabilis causes a large proportion of catheter-associated urinary tract infections (CAUTIs), which are among the world's most common nosocomial infections. Here, we characterize P. mirabilis bacteriophage Privateer, a prolate podophage of the C3 morphotype isolated from Texas wastewater treatment plant activated sludge. Basic characterization assays demonstrated Privateer has a latent period of ~40 minutes and average burst size around 100. In the 90.7 kb Privateer genome, 43 functions were assigned for the 144 predicted protein-coding genes. Genes encoding DNA replication proteins, DNA modification proteins, four tRNAs, lysis proteins, and structural proteins were identified. Cesium-gradient purified Privateer particles analyzed via LC-MS/MS verified the presence of several predicted structural proteins, including the major capsid protein and a longer, minor capsid protein apparently produced by translational frameshift. Comparative analysis demonstrated Privateer shares 83% nucleotide similarity with Cronobacter phage vB_CsaP_009, but low nucleotide similarity with other known phages. Predicted structural proteins in Privateer appear to have evolutionary relationships with other prolate podophages, in particular the Kuraviruses. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-11-29 |
| AnnouncementXML | Submission_2020-11-29_15:45:11.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Susan T. Weintraub |
| SpeciesList | scientific name: Proteus phage Privateer; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | instrument model |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-10-02 13:21:36 | ID requested | |
| ⏵ 1 | 2020-11-29 15:45:12 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: bacteriophage, genomics, proteus |
Contact List
| Ryland Young, Ph.D. | |
|---|---|
| contact affiliation | Texas A&M University |
| contact email | ryland@tamu.edu |
| lab head | |
| Susan T. Weintraub | |
| contact affiliation | UT Health Science Center San Antonio |
| contact email | weintraub@uthscsa.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086232/ |
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