PXD021798-1

PXD021798 is an original dataset announced via ProteomeXchange.

Title	Function and Regulation of the pyruvate transporter CstA in Escherichia coli
Description	Pyruvate is a central metabolite that connects many metabolic pathways in living organisms. To meet the cellular pyruvate requirements, Escherichia coli most likely has three pyruvate uptake systems, BtsT, YhjX and CstA. Transport studies in intact cells proved that CstA is a high specific pyruvate transporter with moderate affinity, which is energized by a proton gradient. When the cells of a reporter strain were cultured in complex medium, cstA expression was bimodal, with the maximum being reached in the stationary phase. A DNA affinity capture assay combined with mass spectrometry and an in vivo reporter assay identified Fis as repressor in addition to the known cAMP-CRP as activator for cstA expression.
HostingRepository	PRIDE
AnnounceDate	2020-12-02
AnnouncementXML	Submission_2020-12-02_04:35:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-02 04:18:32	ID requested
⏵ 1	2020-12-02 04:35:32	announced

Dataset with its publication pending

submitter keyword: pyruvate uptake, global regulator Fis, stationary phase, catabolite repression

Prof. Dr. Kirsten Jung
contact affiliation	Ludwig-Maximilians-Universität München Biozentrum Department Biologie I, Ber. Mikrobiologie Großhaderner Str. 2-4 82152 Martinsried Raum: E 01.024 Telefon: +49 (0)89 / 2180-74500 Fax: +49 (0)89 / 2180-74520
contact email	jung@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD021798

PRIDE project URI

• PRIDE
  1. PXD021798
     1. Label: PRIDE project
     2. Name: Function and Regulation of the pyruvate transporter CstA in Escherichia coli