PXD021782 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of N-linked glycosylated peptides in the open ocean diatom Thalassiosira oceanica |
Description | N-linked glycosylation is a posttranslational modification affecting protein folding and protein function. The N-linked glycosylation pathway in algae is poorly characterized, and further knowledge is needed to understand the cell biology of algae and the evolution of N-linked glycosylation. This study investigated the N-linked glycosylation pathway in the open ocean diatom Thalassiosira oceanica and identified N-linked glycosylated peptides through solid-phase extraction of N-linked glycosylated peptides (SPEG). We characterized 118 N-linked glycosylated peptides from samples of cells grown in high- and low-iron conditions. The identified peptides had 81% NXT-type motifs, with X being any amino acids except proline. The presence of N-linked glycosylation sites in the iron starvation-induced protein 1a (ISIP1a) confirmed its predicted topology, contributing to the biochemical characterization of ISIP1 proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_07:30:29.571.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alejandro Cohen |
SpeciesList | scientific name: Thalassiosira oceanica; NCBI TaxID: 159749; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-10-02 00:40:57 | ID requested | |
⏵ 1 | 2021-09-09 07:30:30 | announced | |
Publication List
Behnke J, Cohen AM, LaRoche J, N-linked glycosylation enzymes in the diatom Thalassiosira oceanica exhibit a diel cycle in transcript abundance and favor for NXT-type sites. Sci Rep, 11(1):3227(2021) [pubmed] |
Keyword List
submitter keyword: N-linked glycosylation, Diatom, Solid-phase extraction of N-linked glycosylated peptides |
Contact List
Julie LaRoche |
contact affiliation | Professor. Tier 1 Canada Research Chair in Marine Microbial Genomics and Biogeochemistry. Department of Biology, Dalhousie University |
contact email | Julie.Laroche@Dal.Ca |
lab head | |
Alejandro Cohen |
contact affiliation | Dalhousie University. Biological Mass Spectrometry Core Facility |
contact email | alejandro.cohen@dal.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD021782
- Label: PRIDE project
- Name: Identification of N-linked glycosylated peptides in the open ocean diatom Thalassiosira oceanica