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PXD021779-1

PXD021779 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome dynamics during cisplatin exposure in sensitive and resistant human lung cancer cells
DescriptionLung cancer is the leading cause of cancer-related deaths and its treatment is based in chemotherapy using platinum containing compounds, mainly cisplatin (CDDP). Many patients show resistance to CDDP leading to treatment failure. To understand the mechanisms involved in CDDP resistance in lung cancer, we used CDDP-sensitive (A549) and –resistant (A549/CDDP) cells to identify newly synthesized proteins in response to CDDP treatment using BONCAT technique. In addition the steady-state proteome of A549 and A549/CDDP cells was also evaluated. It was identified 70 and 69 proteins upregulated by CDDP in A549 and A549/CDDP cells, respectively. The set of proteins upregulated by CDDP in both cells are associated to GO terms related to proteostasis, telomere maintenance cell, RNA processing, cytoskeleton and response to oxidative stress. Interestingly, the profile of biological processes enriched in A549 cells after CDDP treatment is very similar to those identified in the steady-state proteome of A549/CDDP cells, suggesting their positive selection in CDDP-resistant cells development. Therefore, this study of proteomic response to CDDP is relevant to the identification of potential protein targets to development of therapeutic strategies to block drug resistance pathways.
HostingRepositoryPRIDE
AnnounceDate2024-10-08
AnnouncementXMLSubmission_2024-10-08_11:15:49.225.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCristine Dutra
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentXevo G2-XS Tof
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-01 22:55:25ID requested
12024-10-08 11:15:50announced
Publication List
DE Souza Dutra C, Martello CL, Cadore NA, Ferreira HB, Zaha A, Monteiro KM, Proteomic Analysis of the Non-genetic Response to Cisplatin in Lung Cancer Cells. Cancer Diagn Progn, 1(3):235-243(2021) [pubmed]
10.21873/cdp.10032;
Keyword List
submitter keyword: proteomics, drug resistance,BONCAT, cisplatin, lung cancer
Contact List
Karina Mariante Monteiro
contact affiliationLaboratorio de Genomica Estrutural e Funcional Departamento de Biologia Molecular e Biotecnologia Instituto de Biociencias Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil
contact emailkarina@cbiot.ufrgs.br
lab head
Cristine Dutra
contact affiliationUniversidade Federal do Rio Grande do Sul
contact emailcristinedutra@hotmail.com
dataset submitter
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