PXD021779-1

PXD021779 is an original dataset announced via ProteomeXchange.

Title	Proteome dynamics during cisplatin exposure in sensitive and resistant human lung cancer cells
Description	Lung cancer is the leading cause of cancer-related deaths and its treatment is based in chemotherapy using platinum containing compounds, mainly cisplatin (CDDP). Many patients show resistance to CDDP leading to treatment failure. To understand the mechanisms involved in CDDP resistance in lung cancer, we used CDDP-sensitive (A549) and –resistant (A549/CDDP) cells to identify newly synthesized proteins in response to CDDP treatment using BONCAT technique. In addition the steady-state proteome of A549 and A549/CDDP cells was also evaluated. It was identified 70 and 69 proteins upregulated by CDDP in A549 and A549/CDDP cells, respectively. The set of proteins upregulated by CDDP in both cells are associated to GO terms related to proteostasis, telomere maintenance cell, RNA processing, cytoskeleton and response to oxidative stress. Interestingly, the profile of biological processes enriched in A549 cells after CDDP treatment is very similar to those identified in the steady-state proteome of A549/CDDP cells, suggesting their positive selection in CDDP-resistant cells development. Therefore, this study of proteomic response to CDDP is relevant to the identification of potential protein targets to development of therapeutic strategies to block drug resistance pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:15:49.225.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristine Dutra
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Xevo G2-XS Tof

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-01 22:55:25	ID requested
⏵ 1	2024-10-08 11:15:50	announced

DE Souza Dutra C, Martello CL, Cadore NA, Ferreira HB, Zaha A, Monteiro KM, Proteomic Analysis of the Non-genetic Response to Cisplatin in Lung Cancer Cells. Cancer Diagn Progn, 1(3):235-243(2021) [pubmed]

10.21873/cdp.10032;

submitter keyword: proteomics, drug resistance,BONCAT, cisplatin, lung cancer

Karina Mariante Monteiro
contact affiliation	Laboratorio de Genomica Estrutural e Funcional Departamento de Biologia Molecular e Biotecnologia Instituto de Biociencias Universidade Federal do Rio Grande do Sul Porto Alegre Rio Grande do Sul Brazil
contact email	karina@cbiot.ufrgs.br
lab head
Cristine Dutra
contact affiliation	Universidade Federal do Rio Grande do Sul
contact email	cristinedutra@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD021779

PRIDE project URI

• PRIDE
  1. PXD021779
     1. Label: PRIDE project
     2. Name: Proteome dynamics during cisplatin exposure in sensitive and resistant human lung cancer cells