PXD021778-1

PXD021778 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative evaluation of proteomic methods for extracellular matrix characterization
Description	Identification and evaluation of proteins in the extracellular matrix (ECM) has proved challenging due to the insolubility of the bulk of ECM proteins in traditional proteomic extraction buffers. Multiple ECM-focused proteomic workflows have been developed over the past 10 years. These methods rely on decellularization followed by chaotrope extraction and ultimately enzymatic or chemical digestion. To date, there has not been a side-by-side comparison of all current methods on uniform, complex tissue samples. Herein, we present an evaluation of ECM-specific methods alongside several single-shot methods for tissue analysis. We provide recommendations for method selection based on ECM protein identification and sequence coverage as well as the ease and precision of the given methods.
HostingRepository	MassIVE
AnnounceDate	2021-04-30
AnnouncementXML	Submission_2021-04-30_14:02:08.390.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maxwell McCabe
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Deamidated; Oxidation; Acetyl; Gln->pyro-Glu; Carbamyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos; instrument model

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-01 19:42:07	ID requested
⏵ 1	2021-04-30 14:02:08	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: extracellular matrix, proteomics, sample preparation methods, collagen, glycoprotein, proteoglycan, matrisome

submitter keyword: extracellular matrix, proteomics, sample preparation methods, collagen, glycoprotein, proteoglycan, matrisome

Contact List

Kirk Hansen
contact affiliation	University of Colorado Anschutz Medical Campus
contact email	kirk.hansen@ucdenver.edu
lab head
Maxwell McCabe
contact affiliation	University of Colorado
contact email	maxwell.mccabe@cuanschutz.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086224/