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PXD021739-1

PXD021739 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConsequences of fibroblast ablation in adult murine hearts
DescriptionFibroblasts produce the majority of collagen in the heart and are thought to regulate extracellular matrix (ECM) turnover. However, the in vivo role of fibroblasts in structuring the basal ECM network is poorly understood. To examine the effects of fibroblast loss on the microenvironment in the adult murine heart, we generated mice with reduced fibroblast numbers and evaluated the tissue microenvironment during homeostasis and after injury. We determined that a 60-80% reduction in fibroblasts numbers did not overtly change the fibrillar collagen network but did alter the distribution and abundance of type VI collagen, a microfibrillar collagen that forms an open network with the basement membrane. In fibroblast ablated mice, myocardial infarction did not result in ventricular wall rupture, and heart function was more effectively preserved during angiotensin II/phenylephrine (AngII/PE) induced fibrosis. Analysis of cardiomyocyte contractility demonstrated weaker contractions and slower calcium release and reuptake in uninjured and AngII/PE infused fibroblast ablated animals. Moreover, fibroblast ablated hearts have a similar gene expression prolife to hearts with exercise-induced and physiological hypertrophy after AngII/PE infusion. These results suggest that hearts are resilient to a significant degree of fibroblast loss and that fibroblasts can directly impact cardiomyocyte function. Furthermore, a reduction in fibroblasts may have cardioprotective effects heart after injury suggesting that manipulation of the number of fibroblasts may have therapeutic value.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:35:40.153.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD021739
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSumit Bhutada
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; deamidated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-29 05:26:28ID requested
12024-10-07 13:35:41announced
Publication List
Kuwabara JT, Hara A, Bhutada S, Gojanovich GS, Chen J, Hokutan K, Shettigar V, Lee AY, DeAngelo LP, Heckl JR, Jahansooz JR, Tacdol DK, Ziolo MT, Apte SS, Tallquist MD, fibroblast reduction in adult murine hearts. Elife, 11():(2022) [pubmed]
10.7554/elife.69854;
10.6019/PXD021739;
Keyword List
submitter keyword: Fibroblast Ablated, TAILS, Hearts,Murine, N-terminomics
Contact List
Suneel Apte
contact affiliationCleveland Clinic
contact emailaptes@ccf.org
lab head
Sumit Bhutada
contact affiliationCleveland Clinic
contact emailbhutads@ccf.org
dataset submitter
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Dataset FTP location
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