PXD021685-1

PXD021685 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 Evades immune detection in Alveolar Macrophages
Description	Respiratory infections, like the current pandemic SARS-CoV-2 virus, target the epithelial cells in the respiratory tract. However, alveolar macrophages (AMs) are tissue-resident macrophages located within the alveoli of the lung and they play a key role in the early phases of an immune response to respiratory infections. We expect that AMs are the first immune cells to encounter the SARS-CoV-2 and therefore their reaction to SARS-CoV-2 infection will have a profound impact upon the outcome of the infection. Interferons (IFNs) are antiviral cytokines and the first cytokine produced upon viral infection. Here, we challenge AMs with SARS-CoV-2 and to our surprise find that the AMs are incapable of recognising SARS-CoV-2 and produce IFN. This is in contrast to respiratory pathogens, such as influenza A virus and Sendai virus. Callenge of AMs with those viruses resulted in a robust IFN response. The absence of IFN production by AMs upon challenge could explain the initial asymptotic phase of SARS-CoV-2 infections and argues against the AMs as the source of proinflamatory cytokines later in infection.
HostingRepository	PRIDE
AnnounceDate	2020-10-20
AnnouncementXML	Submission_2020-10-19_22:09:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021685
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ebbe Toftgaard Poulsen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-25 02:40:29	ID requested
⏵ 1	2020-10-19 22:09:35	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2

submitter keyword: SARS-CoV-2, PRM, Alveolar macrophages

Jan J. Enghild
contact affiliation	Department of Molecular Biology and Genetics, Aarhus University, Denmark.
contact email	jje@mbg.au.dk
lab head
Ebbe Toftgaard Poulsen
contact affiliation	Department of Molecular Biology and Genetics
contact email	etp@mb.au.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021685
     1. Label: PRIDE project
     2. Name: SARS-CoV-2 Evades immune detection in Alveolar Macrophages