PXD021684-1

PXD021684 is an original dataset announced via ProteomeXchange.

Title	Proteomic Adaptation of Clostridioides difficile to Treatment with the Antimicrobial Peptide Nisin
Description	Clostridioides difficile is the leading cause of antibiotics-associated diarrhea but can also result in more serious, life-threatening conditions. As incidence of C. difficile infections in hospitals is increasing, both in frequency and severity, and antibiotic-resistant C. difficile strains are advancing, antimicrobial peptides (AMPs) are an interesting alternative to classic antibiotics. Knowledge on the effects of AMPs on C. difficile will therefore not only enhance the knowledge for possible biomedical application but may also provide insights in mechanisms of C. difficile to adapt or counteract AMPs. This study applies state-of-the-art mass spectrometry methods to quantitatively investigate the proteomic response of C. difficile 630∆erm to sublethal concentrations of the AMP nisin allowing to follow the cellular stress adaptation in a time-resolved manner. The results do not only point at a heavy reorganization of the cellular envelop but also determined pronounced changes in central cellular processes such as carbohydrate metabolism. Moreover, the number of flagella per cell was changed during the process of adaptation to nisin suggesting a role of these cellular structures in combating this particular AMP, which is independent from pure cell motility
HostingRepository	PRIDE
AnnounceDate	2021-02-18
AnnouncementXML	Submission_2021-02-18_06:12:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Clostridioides difficile 630; NCBI TaxID: 272563;
ModificationList	monohydroxylated residue
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-25 02:40:27	ID requested
⏵ 1	2021-02-18 06:12:58	announced

Dataset with its publication pending

submitter keyword: Nisin, antimicrobial peptides, antibiotic resistance, mass spectrometry, C. difficile, proteomics

Dörte Becher
contact affiliation	University of Greifswald, Institute of Microbiology, Department of Microbial Proteomics, Felix-Hausdorff-Str. 8, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD021684

PRIDE project URI

• PRIDE
  1. PXD021684
     1. Label: PRIDE project
     2. Name: Proteomic Adaptation of Clostridioides difficile to Treatment with the Antimicrobial Peptide Nisin