PXD021675-1

PXD021675 is an original dataset announced via ProteomeXchange.

Title	Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum
Description	Clavulanic acid (CLV) is a betalactam (BL) which inhibits betalactamases activity and is frequently administered combined with amoxicillin (AX). Both BLs can be independently involved in allergic reactions. Indeed, selective immediate allergic reactions to CLV have recently been reported in 30% of patients allergic to the AX-CLV combination. Although protein haptenation with β-lactams is considered necessary to activate the immune system, currently there are no straight-forward detection tools for the study of protein haptenation by CLV. The aim of this study was to assess the suitability of two biotinylated analogues of CLV, CLV-B or CLV-TEG-B (containing the later a hydrophilic tetraethylenglycol linker), as probes to study protein haptenation by this β-lactam. Our results show that tagged CLV keeps some of the features of CLV, as the amide binding by which they haptenate proteins and it could constitute a valuable tool to identify protein targets for haptenation by CLV with high sensitivity to get insights into the activation of the immune system by CLV as well as mechanisms involved in allergy to β-lactams.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_08:27:32.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Plus MALDI TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-24 22:41:22	ID requested
⏵ 1	2021-09-09 08:27:33	announced

Mart, í, n-Serrano Á, Gonzalez-Morena JM, Barbero N, Ariza A, S, á, nchez G, ó, mez FJ, P, é, rez-Inestrosa E, P, é, rez-Sala D, Torres MJ, Monta, ñ, ez MI, Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum: Implications in Allergy Studies. Front Pharmacol, 11():594755(2020) [pubmed]

submitter keyword: Blot and biotin detection, clavulanic acid, biotin tag, biotinylation, clavulanate, haptenation

María Isabel Montañez Vega
contact affiliation	Grupo de Investigación de Enfermedades Alérgica a Alérgenos y Fármacos - Unidad de Gestión clínica de Alergología,Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain
contact email	maribelmv.uma@gmail.com
lab head
Concha Gil
contact affiliation	UMC
contact email	cai.proteomica@pas.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021675

PRIDE project URI

• PRIDE
  1. PXD021675
     1. Label: PRIDE project
     2. Name: Biotin-Labelled Clavulanic Acid to Identify Proteins Target for Haptenation in Serum