PXD021673-1

PXD021673 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of lesional and normally looking psoriatic skin
Description	Background: Plaque psoriasis is a chronic autoimmune disorder characterized by the development of red scaly plaques. To date psoriasis lesional skin transcriptome has been extensively studied, whereas only few proteomic studies of psoriatic skin are available. Aim: The aim of this study was to compare protein expression patterns of lesional and normally looking skin of psoriasis patients with skin of the healthy volunteers, reveal differentially expressed proteins and identify changes in cell metabolism caused by the disease. Methods: Skin samples of normally looking and lesional skin donated by psoriasis patients (n = 5) and samples of healthy skin donated by volunteers (n = 5) were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After protein identification and data processing, the set of differentially expressed proteins was subjected to protein ontology analysis to characterize changes in biological processes, cell components and molecular functions in the patients' skin compared to skin of the healthy volunteers. Results: The performed analysis identified 405 and 59 differentially expressed proteins in lesional and normally looking psoriatic skin compared to healthy control. We discovered decreased expression of KNG1, APOE, HRG, THBS1 and PLG in normally looking skin of the patients. Presumably, these changes were needed to protect the epidermis from spontaneous activation of kallikrein-kinin system and delay the following development of inflammatory response. In lesional skin, we identified several large groups of proteins with coordinated expression. Mainly, these proteins were involved in different aspects of protein and RNA metabolism, namely ATP synthesis and consumption; intracellular trafficking of membrane-bound vesicles, pre-RNA processing, translation, chaperoning and degradation in proteasomes/immunoproteasomes. Conclusion: Our findings explain the molecular basis of metabolic changes caused by disease in skin lesions, such as faster cell turnover and higher metabolic rate. They also indicate on downregulation of kallikrein-kinin system in normally looking skin of the patients that would be needed to delay exacerbation of the disease.
HostingRepository	PRIDE
AnnounceDate	2021-05-06
AnnouncementXML	Submission_2021-05-05_21:53:53.888.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rustam Ziganshin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-24 07:26:10	ID requested
⏵ 1	2021-05-05 21:53:54	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS, proteome, plaque psoriasis

Oxana A. Svitich
contact affiliation	Laboratory of Molecular Immunology, I. Mechnikov Research Institute for Vaccines and Sera Director of the I. Mechnikov Research Institute for Vaccines and Sera
contact email	svitichoa@yandex.ru
lab head
Rustam Ziganshin
contact affiliation	Shemyakin-Ovchinnikov Institute of bioorganic chemistry Russian Academy of Sciences
contact email	rustam.ziganshin@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021673
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of lesional and normally looking psoriatic skin