PXD021649-1

PXD021649 is an original dataset announced via ProteomeXchange.

Title	Online electrochemical reduction of both inter- and intramolecular disulfide bridges in immunoglobulins
Description	Cysteine disulfide bridges can be reduced by chemical methods, like an incubation with DTT. However, reduction with an electrochemical method has also been demonstrated. Electrochemical reduction can be implemented online, after LC separation and before mass spectrometry. For the study of antibody molecules, reduction of the intermolecular disulfide bridges between heavy and light chains has been demonstrated in literature. However, the reduction of intramolecular disulfide bridges has remained elusive. Here, we demonstrate the online electrochemical reduction of a therapeutic monoclonal antibody. The complete reduction of these molecules will facilitate the study of recombinant or natural antibodies by MS and MS/MS.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_02:19:22.695.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martijn van Duijn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-23 08:26:00	ID requested
⏵ 1	2022-05-27 02:19:23	announced

Vanduijn MM, Brouwer HJ, Sanz de la Torre P, Chervet JP, Luider TM, Online Electrochemical Reduction of Both Inter- and Intramolecular Disulfide Bridges in Immunoglobulins. Anal Chem, 94(7):3120-3125(2022) [pubmed]

submitter keyword: Electrochemical reduction

disulfide

top-down

antibody

Theo Marten Luider
contact affiliation	Lab head clinical and cancer proteomics Department of Neurology Erasmus MC, Rotterdam
contact email	t.luider@erasmusmc.nl
lab head
Martijn van Duijn
contact affiliation	Erasmus MC
contact email	m.m.vanduijn@erasmusmc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021649
     1. Label: PRIDE project
     2. Name: Online electrochemical reduction of both inter- and intramolecular disulfide bridges in immunoglobulins