PXD021646-1

PXD021646 is an original dataset announced via ProteomeXchange.

Title	The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes
Description	STRIPAK is a highly conserved eukaryotic signalling complex controlling a wide range of developmental processes in microorganisms as well as humans. This study uses iTRAQ-based proteomic and phosphoproteomic analyses to expand the identification of differentially phosphorylated proteins, comparing the wildtype proteome of the model fungus S. macorspora to those from three STRIPAK single- and double deletion mutants. Among the differentially phosphorylated proteins, we detected Gul1, a homolog of GUL-1 from N. crassa and SSD1 from yeast. In this study, we further provide a functional analysis of Gul1, showing it controls sexual development and hyphal morphology in a phosphorylation dependent manner.
HostingRepository	PRIDE
AnnounceDate	2020-09-24
AnnouncementXML	Submission_2020-09-24_01:50:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bernhard Blank-Landeshammer
SpeciesList	scientific name: Sordaria macrospora k-hell; NCBI TaxID: 771870;
ModificationList	phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-23 06:56:51	ID requested
⏵ 1	2020-09-24 01:50:32	announced

Dataset with its publication pending

submitter keyword: Sordaria macrospora, LC-MSMS, Phosphoproteomics, iTRAQ

Prof. Dr. Albert Sickmann
contact affiliation	Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V.
contact email	sickmann@isas.de
lab head
Bernhard Blank-Landeshammer
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS e.V.
contact email	blank.bernhard@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021646
     1. Label: PRIDE project
     2. Name: The STRIPAK signaling complex regulates dephosphorylation of GUL1, an RNA-binding protein that shuttles on endosomes