PXD021584-1

PXD021584 is an original dataset announced via ProteomeXchange.

Title	Comparative analysis of systemic and tumor microenvironment proteomes of children with b-cell acute lymphocytic leukemia at diagnosis and after induction treatment.
Description	Among the childhood diseases, B-cell acute lymphocytic leukemia (B-ALL) is the most frequent type of cancer. In spite of recent advances concerning disease treatment, cytotoxic chemotherapy remains as the first line of treatment in several countries, and the modifications induced by such drugs in the organism remains poorly understood. In this context, the present study provided a comparative high-throughput proteomic analysis of the cumulative changes induced by chemotherapeutic drugs used in the induction phase of B-LLA treatment in both peripheral blood (PB) and bone marrow compartment (BM) samples. To reach this goal, PB and BM plasma samples were comparatively analyzed by using label-free proteomics at two endpoints: at diagnosis (D0) and the end of the cumulative induction phase treatment (D28). The resulting differentially expressed proteins were explored by bioinformatics approaches aiming to identify the main gene ontology processes, pathways and transcription factors altered by chemotherapy, as well to understand B-LLA biology in each compartment at D0. At D0, PB was characterized as a pro-inflammatory environment, with the involvement of several downregulated coagulation proteins as KNG, plasmin and plasminogen. D28 was characterized predominantly by immune response-related processes, and the super expression of the transcription factor IRF3 and transthyretin. RUNX1 was pointed out as a common transcription factor found in both D0 and D28. Considering that most of these proteins were not described in B-ALL literature, these findings added to understanding disease biology at diagnosis, and highlighted some important proteins and processes that may contribute to our understanding about the mechanisms concerning the impact of chemotherapy in disease resolution.
HostingRepository	PRIDE
AnnounceDate	2020-12-14
AnnouncementXML	Submission_2020-12-14_05:48:23.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021584
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eliana Abdelhay
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	Synapt HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-21 00:48:25	ID requested
⏵ 1	2020-12-14 05:48:23	announced

Dataset with its publication pending

submitter keyword: B-acute lymphocytic leukemia, chemotherapy, proteomics, induction phase.

Carolina Panis
contact affiliation	Universidade Estadual do Oeste do Paraná, Reitoria, Campus Universitário de Francisco Beltrão. Rua Maringa 1200 - Centro de Ciências da Saúde centro 86010000 - Francisco Beltrão, PR - Brasil fone. +55 46 35204886
contact email	carolpanis@hotmail.com
lab head
Eliana Abdelhay
contact affiliation	Pesquisador Senior Divisão de Laboratórios Centro de Transplante de Medula Óssea Instituto Nacional de Câncer Praça da Cruz Vermelha 23 6 andar ala C Centro, Rio de Janeiro/RJ, Brasil
contact email	eabdelhay@inca.gov.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021584
     1. Label: PRIDE project
     2. Name: Comparative analysis of systemic and tumor microenvironment proteomes of children with b-cell acute lymphocytic leukemia at diagnosis and after induction treatment.