PXD021575 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A proteomics-based method for identifying antigens within immune complexes |
Description | A novel approach to recover and identify immune complexes (ICs) was developed using size exclusion chromatography (SEC) and affinity chromatography on immunoglobulin binding columns (HiTrap Protein G). The purification process was monitored by 1D SDS-PAGE, protein staining, Western blotting and, finally, liquid chromatography tandem mass spectrometry (LC MS/MS) was used to identify the recovered antigens. This approach was applied to serum with artificially created immune complexes (ICs) comprising vaccine antigen (influenza) and antibody, which led to recovery and identification of influenza peptides within the recovered ICs. This approach was compared with the established method for IC detection and recovery, polyethylene glycol (PEG) precipitation, followed by LC MS/MS. Both approaches successfully enabled capture, recovery and characterization of immunoglobulins and influenza antigen(s) in complex with the immunoglobulins. However, PEG precipitation has the advantage of simplicity and is more suited for large scale studies. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-04 |
AnnouncementXML | Submission_2021-01-04_02:57:25.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021575 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Andrew McArdle |
SpeciesList | scientific name: Alphainfluenzavirus; NCBI TaxID: 197911; scientific name: Betainfluenzavirus; NCBI TaxID: 197912; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-20 23:38:47 | ID requested | |
⏵ 1 | 2021-01-04 02:57:26 | announced | |
Publication List
Menikou S, McArdle AJ, Li MS, Kaforou M, Langford PR, Levin M, A proteomics-based method for identifying antigens within immune complexes. PLoS One, 15(12):e0244157(2020) [pubmed] |
Keyword List
submitter keyword: Immune complexes, Metaproteomics, Human, Influenza, Orbitrap |
Contact List
Michael Levin |
contact affiliation | Section of Paediatric Infectious Disease |
contact email | m.levin@imperial.ac.uk |
lab head | |
Andrew McArdle |
contact affiliation | Imperial College London |
contact email | a.mcardle@imperial.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD021575
- Label: PRIDE project
- Name: A proteomics-based method for identifying antigens within immune complexes