PXD021543 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Flavonol-mediated stabilization of efflux complexes regulates polar auxin transport |
Description | The transport of auxin controls the rate, direction and localization of plant growth and development. The course of auxin transport is defined by the polar subcellular localization of the PIN proteins, a family of auxin efflux transporters. Using blue native PAGE and quantitative mass spectrometry, we identify native PIN core transport units as homo- and heteromers assembled from PIN1, 2, 3, 4 and 7 subunits only. To identify the protein composition of the larger, low stringency-solubilized PIN assembly we affinity-purified GFP-fused PIN proteins from Arabidopsis roots after expression from their native promoters and after the induction of discrete lateral roots, and analyzed the captured proteins by label-free quantitative mass spectrometry (LC-MS/MS). |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-08 |
AnnouncementXML | Submission_2020-10-07_23:00:54.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD021543 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Haupt |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | S-carboxamidoethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-17 08:37:06 | ID requested | |
⏵ 1 | 2020-10-07 23:00:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Arabidopsis thaliana, auxin transport, AP-MS |
Contact List
Prof. Dr. Bernd Fakler |
contact affiliation | Institute of Physiology II Universität Freiburg Germany |
contact email | bernd.fakler@physiologie.uni-freiburg.de |
lab head | |
Alexander Haupt |
contact affiliation | University of Freiburg, Germany |
contact email | alexander.haupt@physiologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021543
- Label: PRIDE project
- Name: Flavonol-mediated stabilization of efflux complexes regulates polar auxin transport