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PXD021543-1

PXD021543 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFlavonol-mediated stabilization of efflux complexes regulates polar auxin transport
DescriptionThe transport of auxin controls the rate, direction and localization of plant growth and development. The course of auxin transport is defined by the polar subcellular localization of the PIN proteins, a family of auxin efflux transporters. Using blue native PAGE and quantitative mass spectrometry, we identify native PIN core transport units as homo- and heteromers assembled from PIN1, 2, 3, 4 and 7 subunits only. To identify the protein composition of the larger, low stringency-solubilized PIN assembly we affinity-purified GFP-fused PIN proteins from Arabidopsis roots after expression from their native promoters and after the induction of discrete lateral roots, and analyzed the captured proteins by label-free quantitative mass spectrometry (LC-MS/MS).
HostingRepositoryPRIDE
AnnounceDate2020-10-08
AnnouncementXMLSubmission_2020-10-07_23:00:54.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD021543
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Haupt
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListS-carboxamidoethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-17 08:37:06ID requested
12020-10-07 23:00:55announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis thaliana, auxin transport, AP-MS
Contact List
Prof. Dr. Bernd Fakler
contact affiliationInstitute of Physiology II Universität Freiburg Germany
contact emailbernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliationUniversity of Freiburg, Germany
contact emailalexander.haupt@physiologie.uni-freiburg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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