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PXD021538-1

PXD021538 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTop-down proteomics of human saliva in Alzheimer disease
DescriptionAlzheimer disease (AD) is the most prevalent neurodegenerative disease and form of dementia in the elderly. It is characterized by the accumulation in the brain of misfolded proteins, inflammation and oxidative damage leading to region-specific loss of synaptic contacts and neuronal cell death. In the present study, we aimed to characterize the salivary protein profile of a sample (sample group or cohort) of AD patients in comparison with healthy controls (HC) by a top-down proteomic approach, to evidence possible quali-/quantitative variations associated to the disease. This approach is based on high performance liquid chromatography separation coupled to electrospray-ion trap mass spectrometry (HPLC-ESI-IT-MS) analysis of the acidic soluble proteome of human saliva, which was standardized in our previous studies for detecting and quantifying of hundreds salivary peptides/proteins. By this, it is possible to obtain a profiling of the naturally occurring salivary proteome, including isoforms and post-translational modifications (PTMs), to compare an unlimited number of samples and to quantify proteins by a label-free method.
HostingRepositoryPRIDE
AnnounceDate2021-05-24
AnnouncementXMLSubmission_2021-05-24_04:11:37.237.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSimone Serrao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList2-pyrrolidone-5-carboxylic acid (Gln); S-nitrosyl-L-cysteine; monoacetylated residue; phosphorylated residue; disulfide crosslinked residues; monohydroxylated residue; O-phospho-L-threonine with neutral loss of phosphate; L-cysteine glutathione disulfide
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-17 08:36:50ID requested
12021-05-24 04:11:37announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Alzheimer disease, salivary proteomics, S100A, cystatins, α-defensins, thymosin β4, antimicrobial peptides, oxidative stress
Contact List
Tiziana Cabras
contact affiliationLife and Environmental Sciences Department (Biomedical Section) - University of Cagliari
contact emailtcabras@unica.it
lab head
Simone Serrao
contact affiliationLife and Environmental Sciences Department (Biomedical Section) - University of Cagliari
contact emailsimone.serrao@unica.it
dataset submitter
Full Dataset Link List
Dataset FTP location
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