PXD021538-1

PXD021538 is an original dataset announced via ProteomeXchange.

Title	Top-down proteomics of human saliva in Alzheimer disease
Description	Alzheimer disease (AD) is the most prevalent neurodegenerative disease and form of dementia in the elderly. It is characterized by the accumulation in the brain of misfolded proteins, inflammation and oxidative damage leading to region-specific loss of synaptic contacts and neuronal cell death. In the present study, we aimed to characterize the salivary protein profile of a sample (sample group or cohort) of AD patients in comparison with healthy controls (HC) by a top-down proteomic approach, to evidence possible quali-/quantitative variations associated to the disease. This approach is based on high performance liquid chromatography separation coupled to electrospray-ion trap mass spectrometry (HPLC-ESI-IT-MS) analysis of the acidic soluble proteome of human saliva, which was standardized in our previous studies for detecting and quantifying of hundreds salivary peptides/proteins. By this, it is possible to obtain a profiling of the naturally occurring salivary proteome, including isoforms and post-translational modifications (PTMs), to compare an unlimited number of samples and to quantify proteins by a label-free method.
HostingRepository	PRIDE
AnnounceDate	2021-05-24
AnnouncementXML	Submission_2021-05-24_04:11:37.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Serrao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); S-nitrosyl-L-cysteine; monoacetylated residue; phosphorylated residue; disulfide crosslinked residues; monohydroxylated residue; O-phospho-L-threonine with neutral loss of phosphate; L-cysteine glutathione disulfide
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-17 08:36:50	ID requested
⏵ 1	2021-05-24 04:11:37	announced

Dataset with its publication pending

submitter keyword: Alzheimer disease, salivary proteomics, S100A, cystatins, α-defensins, thymosin β4, antimicrobial peptides, oxidative stress

Tiziana Cabras
contact affiliation	Life and Environmental Sciences Department (Biomedical Section) - University of Cagliari
contact email	tcabras@unica.it
lab head
Simone Serrao
contact affiliation	Life and Environmental Sciences Department (Biomedical Section) - University of Cagliari
contact email	simone.serrao@unica.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD021538

PRIDE project URI

• PRIDE
  1. PXD021538
     1. Label: PRIDE project
     2. Name: Top-down proteomics of human saliva in Alzheimer disease