PXD021527-1

PXD021527 is an original dataset announced via ProteomeXchange.

Title	Methionyl-tRNA synthetase interactome
Description	During mRNA translation, tRNAs are charged by aminoacyl-tRNA synthetases (aaRS) and subsequently used by ribosomes. A multi-enzyme aminoacyl-tRNA synthetase complex (MSC) has long been proposed to increase protein synthesis efficiency by passing charged tRNAs directly to ribosomes. An alternative is that the MSC repurposes specific synthetases for ex-translational functions that are activated by cues that direct specific enzymes to novel targets. To explore this question, we generated mammalian cell clones in which ArgRS and GlnRS were absent from the MSC to give a stable complex lacking the two enzymes (MSCΔRQ). Protein synthesis, under a variety of stress conditions, was unchanged in MSCΔRQ cells. Most strikingly, levels of charged tRNAGln and tRNAArg remained unchanged and no ribosome pausing was observed at codons for Arg and Gln. Thus, increasing or regulating protein synthesis efficiency is not dependent on ArgRS and GlnRS in the MSC. Alternatively, and consistent with previously reported ex-translational roles, we found manipulations that do not affect protein synthesis but instead MSC cellular localization.
HostingRepository	PRIDE
AnnounceDate	2020-12-23
AnnouncementXML	Submission_2020-12-23_01:56:58.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021527
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Haissi Cui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-17 00:51:48	ID requested
⏵ 1	2020-12-23 01:56:58	announced

Dataset with its publication pending

submitter keyword: multisynthethase complex, aminoacyl-tRNA synthetases

Paul Schimmel
contact affiliation	Scripps Laboratories for tRNA Synthetase Research The Scripps Research Institute 10550 North Torrey Pines Road, BCC379 La Jolla, CA 92037
contact email	schimmel@scripps.edu
lab head
Haissi Cui
contact affiliation	Scripps Research
contact email	hacui@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD021527

PRIDE project URI

• PRIDE
  1. PXD021527
     1. Label: PRIDE project
     2. Name: Methionyl-tRNA synthetase interactome