PXD021515-1

PXD021515 is an original dataset announced via ProteomeXchange.

Title	CD63/CD9/CD63-YA-GFP extracellular vescicles LC-MS/MS
Description	This study aimed to identify subpopulations of extracellular vesicles (EVs) secreted by HeLa cells, and determine markers which enable to identify them, and which indicate their endosomal or plasma membrane origin. We used CD63 and CD9 as markers of EVs, and followed their intracellular trafficking using the RUSH assay. We used mass spectrometry to identify specific or common proteins in immunoprecipitated GFP+ EVs from HeLa transfected with the RUSH CD63-GFP or CD9-GFP, after a short (3h) or a long (24h) trafficking time from the endoplasmic reticulum.
HostingRepository	PRIDE
AnnounceDate	2021-05-17
AnnouncementXML	Submission_2021-05-17_05:08:34.872.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valentin SABATET
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-16 05:05:36	ID requested
⏵ 1	2021-05-17 05:08:35	announced
2	2021-06-23 22:02:15	announced	2021-06-24: Updated project metadata.
3	2021-08-26 04:24:48	announced	2021-06-24: Updated project metadata. 2021-08-26: Updated publication reference for PubMed record(s): 34282141.

Dataset with its publication pending

submitter keyword: Human, extracellular vesicles

Damarys Loew
contact affiliation	Head of the Curie Institute Mass Spectrometry Platform
contact email	damarys.loew@curie.fr
lab head
Valentin SABATET
contact affiliation	Curie Institute
contact email	valentin.sabatet@curie.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD021515

PRIDE project URI

• PRIDE
  1. PXD021515
     1. Label: PRIDE project
     2. Name: CD63/CD9/CD63-YA-GFP extracellular vescicles LC-MS/MS