PXD021506-2

PXD021506 is an original dataset announced via ProteomeXchange.

Title	Label-free shotgun proteomics of Triton X-100 insoluble fractions of Lon and FtsH conditional mutants of Mycoplasma pneumoniae under depleting and inducing conditions
Description	Using Mycoplasma pneumoniae as a model organism, we conditionally depleted the two essential ATP-dependent proteases (Lon and FtsH) of this bacterium, by engineering strains carrying a Lon and/or FtsH inducible expression locus. An integrative comparative study combining label-free shotgun proteomics and RNA-seq allowed us to decipher the global cellular response to Lon and FtsH depletion and to define protease substrates in this genome-reduced organism. This particular dataset corresponds to the analysis of the Triton X-100 insoluble fractions of Lon and FtsH mutants grown under inducing and depleting conditions. The dataset for the whole cell lysate is available under the identifier PXD016343.
HostingRepository	PRIDE
AnnounceDate	2020-11-24
AnnouncementXML	Submission_2021-03-09_01:44:11.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Weber
SpeciesList	scientific name: Mycoplasma pneumoniae M129; NCBI TaxID: 272634;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-16 01:22:37	ID requested
1	2020-11-23 23:04:50	announced
⏵ 2	2021-03-09 01:44:12	announced	2021-03-09: Updated publication reference for PubMed record(s): 33320415.

Burgos R, Weber M, Martinez S, Lluch-Senar M, Serrano L, Protein quality control and regulated proteolysis in the genome-reduced organism Mycoplasma pneumoniae. Mol Syst Biol, 16(12):e9530(2020) [pubmed]

submitter keyword: protease, Mycoplasma pneumoniae, protein homeostasis, degradation, insoluble fraction

Luis Serrano
contact affiliation	Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain
contact email	luis.serrano@crg.eu
lab head
Marc Weber
contact affiliation	Centre for Genomic Regulation
contact email	marc.weber@crg.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD021506

PRIDE project URI

• PRIDE
  1. PXD021506
     1. Label: PRIDE project
     2. Name: Label-free shotgun proteomics of Triton X-100 insoluble fractions of Lon and FtsH conditional mutants of Mycoplasma pneumoniae under depleting and inducing conditions